中文摘要：

目的探讨多药耐药（MDR）基因产物P-糖蛋白（P—gp）、谷胱甘肽-S-转移酶-π（GST-π）及DNA拓扑异构酶Ⅱ（TopoII）在乳腺癌组织中的表达及其相互关系。方法采用免疫组织化学方法，检测184例未经抗肿瘤治疗的原发性乳腺癌组织中P—gP、GST-π及Topo II表达水平。结果乳腺癌组织中P—gP、GST-π和TopoII阳性表达率分别为49．51％、45．65％和73．91％，TopoII阳性表达率明显高于P—gp及GST-π阳性表达率（P〈0．05）；2种多药耐药基因产物共表达率为30．98％，2种和3种多药耐药基因产物共表达率为67．39％，明显高于单独表达率12．41％（P〈0．01）；P—gP与GST-π的表达呈显著正相关（P〈0．05），P—gP和TopoII的表达呈明显负相关（P〈0．05），GST-π和TopoII的表达呈明显负相关（P〈0．05）。结论P—gP、GST-π及TopoII在乳腺癌组织中表达对肿瘤耐药起重要作用，单基因和多基因协同作用，以多基因共表达为主。

英文摘要：

Objective To explore expression of muhidrug resistance （MDR） gene production of P - glycoprotein （ P - gp）, Glntathione - s - transferase - π （ GST - π） and Topo II in breast cancer tissue. Methods A total of 184 case with untreated primary breast carcinoma were detected immunohistochemically for P - gp, GST - π and Topo II. Results The positive expression rate of P - gp, GST - π and Topo II separately were 49.51% ,45.65% and 73.91%. The positive expression rate of Topo II was much higher than that of P- gp,GST-π （ P 〈 0.05）. The coexpression rate of only 2 MDR gene production and 3 MDR gene production was 30.98% and 67.7% , respectively. Those coexpression rates were higher than individual expression rate （ 12.41% ）. There was significantly pos- itive correlation beteen P - gp and GST - π （ P 〈 0.05） and there was significantly negative correlation beteen P - gp related and Topo II （P 〈 0.05 ）. GST - π had obviously negative correlation with Topo II （ P 〈 0.05 ）, Conclusion The expression of P - gp, GST -π and Topo 11 in breast cancer tissue play an important role in tumor drug - resistance. There is synergistic action between single gene and multiple genes.