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文昌鱼的实验室繁育及子二代获得
  • 期刊名称:动物学研究,27(6):631-634,2006
  • 时间:0
  • 分类:Q959.287[生物学—动物学]
  • 作者机构:[1]厦门大学生命科学学院,福建厦门361005, [2]福建师范大学生命科学学院,福建福州350007, [3]福建海洋研究所,福建厦门361012
  • 相关基金:国家自然科学基金(30470938和30570208);福建省自然科学基金(D0510002);厦门市科技计划重点项目(3502220042015)资助致谢:参与养殖管理的人员还有本实验室的李光、陈文、陈路和林鲁萍等研究生;福建海洋研究所方少华研究员提供帮助,一并表示感谢.
  • 相关项目:文昌鱼Pax家族基因组结构和胚胎发生过程中的表达分析
中文摘要:

文昌鱼特殊的进化地位、简单的器官系统和终生透明的躯体等特征,使其很有希望成为一个新型实验室模式动物。要实现文昌鱼的模式动物化,实验室内全人工条件下繁殖是关键的第一步。为此,我们于2003年9月和2004年4月两次采集产于厦门海域的2种文昌鱼,开展实验室内养殖研究。经过3年多的持续实验室养殖,继2005年夏季于实验室内繁殖出子一代文昌鱼后,又在2006年夏季成功获得了这两种文昌鱼的子二代,初步实现文昌鱼在实验室内的全人工养殖。对子代文昌鱼养殖的初步观察发现,不同水温对生长发育速度有一定影响,提示有可能通过水温控制实现文昌鱼一年多次产卵的目的。目前这两种文昌鱼子二代幼体已完成变态,进入亚成体生长发育阶段,其体长分别已达14.6mm(日本文昌鱼Branchiostoma japonicum)和6.5mm(白氏文昌鱼B.belcheri)。

英文摘要:

Amphioxus is expected to be a model laboratory animal in the near future because of its important position in evolution, simplified internal organ systems and transparent body. In order to develop the animal into a new laboratory model, we sampled two species of amphioxus ( Branchiostoma belcheri and B. japonicum ) from Xiamen in September 2003 and April 2004. The captive lancelets were cultured in the laboratory and fed daily with unicellular algae. After three years of laboratory culturing, we successfully obtained a second filial generation of both species, meaning that a full reproductive cycle was achieved in the laboratory. Preliminary observations on the growth of the progeny indicated that warmer water temperature will speed up the growth and development of amphioxus. This hints that amphioxus probably spawns more often in the laboratory, where water temperature is controlled, than in the wild. Most of the second generation lancelets have now metamorphosed into the early sub-adult stage, and their maximum full body lengths were up to 14.6 mm (B. japonicum ) and 6.5 mm ( B. belcheri ), respectively.

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