中文摘要：

目的：采用[11C]N-甲基乙酰唑胺作为PET/CT的显像剂,研究肝脏纤维化不同阶段肝组织摄取率,探讨[^11C]N-甲基乙酰唑胺在评价肝脏纤维化早期诊断的价值。材料与方法：Wistar大鼠26只（雄性）、重量（29 240 g）,对照组大鼠（N=8）,硫代乙酰胺（TAA）成功建立肝纤维化模型大鼠（N=18）,其中轻度肝纤维化（S1,N=10）及肝中重度纤维化（S2＋S3,N=8）。以[^11C]N-甲基乙酰唑胺为显像剂,先对大鼠进行PET/CT动态扫描,选择存活大鼠再以乙酰唑胺为抑制剂进行抑制实验。实验结束后大鼠处死、取材。每次扫描结束后进行数据重建,分别计算不同时间点（20 s、90 s、5 min、10 min、20 min、30 min和45 min）肝脏标准摄取值（SUV）,分析比较其与病理结果之间的联系及相关性。结果：对照组与实验组90 s、5 min、10 min SUV值具有显著性差异;实验组组内各时间点SUV值无显著性差异。实验组SUV值与病理免疫组化结果之间具有良好相关性。对照组抑制实验呈阳性结果,而实验组抑制实验呈阴性结果,且随肝纤维化程度加重,抑制效率减低。结论：[^11C]N-甲基乙酰唑胺PET/CT肝脏显像能够用于检测早期纤维化时功能受损,进而评估肝纤维化的严重程度,对肝脏纤维化分期。

英文摘要：

Objective： The [^11C]N-methyl acetazolamide（[^11C]N-MAC）, as the imaging agent, was used to study the uptake rate of liver tissue in different stages of liver fibrosis by PET/CT, and then to investigate its value in evaluating early diagno- sis of liver fibrosis. Method： Twenty-six Wistar rats（male, 29 240 g weight） were included with 8 in the control group and 18 in the test group （mild liver fibrosis, S1, N=10 and severe liver fibrosis, S2＋S3, N=8）. Liver fibrosis was induced by thioac- etamide（TAA）. After PET/CT dynamic scanning with [HC]N-MAC, the mice which were alive in the second day, continued to receive inhibition test with acetazolamide. Data reconstruction was accomplished after scanning. The standard uptake value （SUV） of the liver at 5 s, 20 s, 90 s, 5 min, 10 min, 20 min, 30 min and 45 min were calculated to analyze its correlation with pathological results. Results： The SUVs at different time points （90 s, 5 min, 10 min） of the control group and test group showed significant correlation. But there was no significant correlation between different stages of liver fibrosis. There was good correlation between the SUV values in the test group and the pathological results of immunohistochemistry. The results of inhi- bition tests were positive for the control group and negative for the test group. With the aggravation of hepatic fibrosis, the inhibiting efficiency decreased. Conclusion： The [^11C]N-MAC PET/CT is a promising tool to detect the early tendency of liver fibrosis by the level of SUV values and then to distinguish the stages of liver fibrosis.