采用温室盆栽试验,研究盐胁迫下,接种摩西球囊霉( Glomus mosseaw)和根内球囊霉( Glomus untrara-dices)对黄檗耐盐能力的影响。结果表明:盐胁迫下,丛枝菌根(AM)真菌对黄檗侵染率影响不显著(p>0.05),黄檗叶片丙二醛( MDA)质量分数显著高于对照处理,接种AM真菌处理的黄檗叶片MDA质量摩尔浓度均显著低于不接种处理( p<0.05);脯氨酸质量分数均较未接种植株升高,AM真菌有效地减轻黄檗受胁迫的程度。接种AM真菌显著增加了可溶性蛋白质量分数,AM真菌增强了黄檗非酶促防御系统能力。盐胁迫下,过氧化物酶( CAT)和过氧化氢酶(POD)活性在菌根和非菌根处理间差异不显著(p>0.05),超氧岐化酶(SOD)活性在菌根和非菌根植株处理间差异显著( p<0.05)。接种AM真菌后,菌根化黄檗植株可通过渗透调节物质积累和抗氧化酶活性的提高,降低体内膜脂过氧化产物的水平,缓解胁迫对黄檗植株的伤害,增强了黄檗对盐胁迫的耐受力。
The experiment was conducted to study the effect of inoculated with Glomus mosseaw and G.untraradices on salt toler-ance ability under salt stress by a pot experiment under greenhouse conditions .The effect of infection rate of Arbuscular my-corrhizal ( AM) fungi under salt stress on Phellodendron amurense has no significance ( p〉0.05) .MDA content in leaves of P.amurense under salt stress was significantly higher than that of the control treatment , and the MDA content in leaves of P.amurense inoculated with two species fungi treatment was significantly lower than that of non -inoculation treatment ( p〈0.05) .The proline content was higher than that of non inoculation treatment , AM fungi can effectively reduce the degree of stress of P.amurense.Inoculated treatment with two species fungi significantly enhanced soluble protein , and AM fungi can enhance non-enzymatic defensive System ability of P.amurense.There were not significantly difference mycorrhizal and non-mycorrhizal treatments of CAT and POD activities under salt stress (p〉0.05), in addition, there was significantly dif-ferent between the mycorrhizal and non-mycorrhizal plants of the SOD activity ( p〈0.05) .After inoculated with AM fungi , mycorrhizal plants of P.amurense can be, through osmoregulation substance accumulation and antioxidant enzyme activity , raised to reduce the level of lipid peroxidation productions , to relieve the stress on the plant damage of P.amurense, and to enhance the salt tolerance of P.amurense.