中文摘要：

目的：了解临床分离的一株荧光假单胞菌的耐药情况和机制。方法：微量稀释法测定细菌对多种抗生素的最低抑菌浓度，双纸片协同法进行金属酶检测，PCR扩增Ⅰ型整合酶及金属酶基因，分光光度计测定酶粗提液对抗生素的稳定性。结果：菌株对β-内酰胺类抗生素和碳青霉烯类抗生素高度耐药，产IMP型金属β-内酰胺酶，酶的编码基因位于Ⅰ型整合子上，酶粗提液对碳青霉烯类抗生素的水解能力与对青霉素的水解能力接近。结论：IMP型金属酶的产生是该菌对β-内酰胺类抗生素耐药的主要原因。

英文摘要：

Objective： To investigate the resistance and its mechnism of a Pseudomonas fluorescens isolate. Methods： Minimum inhibitory concentration（MIC）was determined by micro-dilution broth method. Double-disk synergy test was performed to detect the production of metallo-β-lactamases（MBL）. Class 1 integrase （lntI1）, VIM, IMP and SPM MBL were detected by polymerase chain reaction. Stability to antibiotics was determined using spectrophotometer. Results： The strain was highly resistant to β-lactam and carbapenems. IMP MBL was found as a cassette on a class 1 integron. The capacity of hydrolyzing cabapenmes of crude β-lactamse extract was similar to that of hydrolyzing penicillin. Conclusion： The production of IMP MBL is the reason for the resistance to β-lactam and carbapenems of this isolate.