中文摘要：

目的探讨不同运动量对2型糖尿病大鼠肝脏Toll样受体4（TLR4）和核转录因子-KB（NF—KB）表达的影响。方法将50只SD人选大鼠按随机数字表法分为对照组、糖尿病模型组（DM组）和糖尿病小运动量组（LIE组）、糖尿病中等运动量组（MIE组）、糖尿病大运动量组（HIE组），每组10只。对照组给予普通膳食，其余4组给予高脂高糖膳食并用小剂量链脲佐菌素（STZ）腹腔注射建立2型糖尿病大鼠模型，然后LIE组、MIE组和HIE组分别采用40％、70％和100％的力竭时间进行游泳训练，每日1次，每周6d，共运动干预6周。由于感染和游泳溺水等原因，运动干预结束后大鼠共存活41只，其中对照组10只，DM组8只，LIE组8只，MIE组8只，HIE组7只。运动干预结束后检测大鼠体重、肝重和空腹血糖，采用RT-PCR技术测定肝脏TLR4和NF—kB的mRNA表达，采用免疫组化技术测定肝脏TLR4和NF—kB的蛋白表达。结果①干预6周后，糖尿病模型各组（DM组、LIE组、MIE组、HIE组）大鼠的体重[（265．00±21．00）、（274．00±22．00）、（286．00±14．00）和（271．00±21．00）g]和肝重[（12．64±1．03）、（12．84±0．85）、（12．65±0．98）和（12．11±0．67）g]显著低于对照组[（567．00±26．00）g体重和（18．04±2．25）肝重g]，差异有统计学意义（P〈0．05）；而糖尿病模型各组大鼠的空腹血糖值[（29．51±3．48）、（23．64±2．39）、（15．45±1．36）和（25．54±2．48）mmot／L]显著高于对照组[（5．77±0．57）mmol／L]，差异亦有统计学意义（P〈O．05）；与DM组比较，糖尿病模型各运动组的体重和肝重变化差异无统计学意义（P〉0．05），但各运动组的血糖有不同程度的降低，差异有统计学意义（P〈0．05）；其中MIE组大鼠的空腹血糖值与HIE组和LIE组相比，降低最为显著，组间差异有统计学意义（P〈0．05）。②干预6?

英文摘要：

Objective To investigate the changes in toll-like receptor 4 （TLR4） and nuclear transcription factor-KB （NF-KB） expression in the livers of diabetic rats after exercise at different intensities. Methods Fifty Sprague-Dawley rats were randomly divided into a control group （ NC ）, a diabetic group （ DM ）, a low intensity exer- cise group （LIE）, a medium intensity exercise group （MIE） and a high intensity exercise group （HIE）, each of 10. The control group was given a normal diet. Type 2 diabetes was induced in the others through feeding a high-fat and high-sugar diet, as well as injecting a small dose of streptozotocin intraperitoneally. The three exercising groups swam to 40%, 70% and 100% of their exhaustion times once a day, 6 d/w for 6 weeks. Due to infection, drowning andother reasons, only 8 rats in each of the DM, LIE and MIE groups, as well as 7 in the HIE group survived. After the intervention, body weight, liver weight and fasting blood glucose （FBG） were tested. The expression of TLR4 and NF-KB mRNA in the livers was tested using RT-PCR technology, and the expression of TLR4 and NF-KB protein in the liver was tested using immunohistochemistry. Resalts Compared with the NC group, the average body weight and liver weight of the other four groups decreased significantly, while the FBG and the expression of TLR4 and NF-KB mRNA and protein increased significantly. There were significant differences among the exercise groups with the lowest values in the MIE group. Conclusion Six weeks of aerobic exercise can reduce FBG and the level of ex- pression of TLR4 and NF-KB mRNA and protein in the liver, at least in rats. The effect of exercise at medium intensi- ty is best.