中文摘要：

背景肿瘤有一个能力变得在间充质的干细胞(MSC ) 并且指导 MSC 移居到肿瘤织物充实。但是在在在肿瘤织物嫁接的 MSC 以后的肿瘤生长上有在肿瘤织物和效果的 MSC 的分发和区别上的相关报告的缺乏。在这研究，我们在肿瘤织物和 24 只新西兰兔子随机被分类进控制组和测试组的在本地肿瘤 microenvironment.Methods 的正式就职下面区分进 myofibroblast 的 MSC 的可能性观察了骨头髓 MSC 的分发。MSC 被孤立并且为每个动物有教养， vx-2 肿瘤织物在每个动物的膀胱 mucosa 下面被移植。在移植以后的一个星期，当仅仅 Dulbecco 的修改的鹰的媒介低的葡萄糖被灌输进控制组时， 4',6-diamidino-2-phenylindole 标记的自我 F2 经过 MSC 在测试组被移植进肿瘤组织。Ultrasonography 被执行因为在 vx-2 肿瘤团以后的各个动物 1,2， 3 和 4 星期被移植。每个动物的最大的膀胱肿瘤直径被记录，每个组的吝啬的价值是计算的。从每个组的一个动物在第四个星期内在第三个星期和留下的动物内被牺牲观察肿瘤发展。与测试组一样对待的另一个动物被牺牲一个星期在自我 MSC 移植以后在肿瘤织物观察 MSC 的分发。Immunofluorescence 被用来在肿瘤织物跟踪 MSC。为光滑的肌肉肌动朊的双标记 immunofluorescence (-SMA) 和 vimentin 被执行识别 MSC 是否能区分进 ultrasonography 肿瘤质量一星期在 vx-2 肿瘤团移植以后不显示出的 myofibroblast.Results。控制组和测试组的吝啬的最大的肿瘤直径是(0.70 瘠污敵 ? 景 ???? 眠牥 ? ㄨ ? 7 ??? ???????????????? 朠慲瑦吗？？

英文摘要：

Background Tumor has an ability to become enriched in mesenchymal stem cells （MSCs） and of guiding MSCs to migrate to tumor tissue. But there are lack of relevant reports on the distribution and differentiation of MSCs in tumor tissue and the effect on tumor growth after MSCs engrafted in tumor tissue. In this study, we observed the distribution of bone marrow MSCs in tumor tissue and the possibility of MSCs differentiating into myofibroblast under the induction of local tumor microenvironment. Methods Twenty-four New Zealand rabbits were randomly classified into the control group and the test group. MSCs were isolated and cultured for each animal, vx-2 tumor tissue was transplanted under the bladder mucosa of each animal One week after the transplantation, the self F2 passage MSCs marked by 4＇,6-diamidino-2-phenylindole were transplanted into tumor tissue in the test group while only Dulbecco＇s modified Eagle＇s medium-low glucose was infused into the control group. Ultrasonography was performed for each animal 1, 2, 3 and 4 week（s） after the vx-2 tumor mass was transplanted. The maximum bladder tumor diameter of each animal was recorded and the mean value of each group was calculated. One animal from each group was sacrificed in the third week and the remaining animals in the fourth week to observe the tumor development. Another animal treated the same as the test group was sacrificed to observe the distribution of MSCs in tumor tissue one week after self MSCs transplantation. Immunofluorescence was used to trace MSCs in tumor tissue. The double labeling immunofluorescence for α-smooth muscle actin （α-SMA） and vimentin was performed to identify whether the MSCs can differentiate into myofibroblast. Results The ultrasonography showed no tumor mass one week after the vx-2 tumor mass transplantation. The mean maximum tumor diameter of the control group and test group was （0.70±0.14） cm and （0.78±0.14） cm, respectively, and there was no significant difference （t=1.308, P=0.204）. T