中文摘要：

目的：观察哮喘大鼠骨形态构建蛋白2，4（BMP-2，BMP-4）表达情况。方法：SD大鼠45只，随机分为2组，对照组（Ⅰ组）为健康大鼠；哮喘模型组（Ⅱ组）为卵清白蛋白（OVA）致敏后吸入激发建立的模型。HE染色后图像分析检测大鼠气道形态学参数，免疫组化方法用于BMP-2，BMP．4表达定位，Western蛋白印迹法测定BMP-2，BMP-4含量。结果：Ⅱ组与Ⅰ组相比，支气管内径缩小。平滑肌层增厚（P〈0．05）；BMP-2表达增加12．3％。BMP-4表达减少7．9％（P〈0．05）。结论：哮喘大鼠肺内BMP-2，BMP-4可能参与哮喘病理生理变化调控。

英文摘要：

Objective： To investigate the expressions of bone morphogenetic protein-2 （BMP-2） and BMP-4 in the lungs of rats with asthma. Methods： Forty-five SD rats were randomly divided into2 groups： group 1 （control group） and group 2 （asthma group）. The asthmatic rat model was established by ovalbumin （OVA） sensitization and challenge. The changes of airway, the expressions of BMP- 2 and BMP-4, and the contents of BMP-2 and BMP-4 were determined by using image analysis system,immunohistochemical method, and Western Blotting, respectively. Results： Compared with group 1, the bronchial internal diameter decreased and the thickness of bronchial smooth mascle increased in group 2 （ P 〈 0.05 ）. The expression of BMP-2 was significantly higher than that in group 1 （ 42. 1 ± 6.2vs. 29.8 ± 8.4% , P 〈 0.05 ） , and the expression of BMP-4 in group2 was signiificantlylower than that in group 1 （11.5 ±4.2 vs. 19.4 ±5.3%, P 〈0.05）. Conclusion： The increase in BMP-2 expression and the decrease in BMP-4 expression may participate in the pathogenesis of asthma in rats.