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茉莉酸甲酯和机械损伤对茶树叶片多酚氧化酶时序表达的影响
  • ISSN号:1000-4025
  • 期刊名称:《西北植物学报》
  • 时间:0
  • 分类:Q945.78[生物学—植物学]
  • 作者机构:[1]中国农业科学院茶叶研究所,杭州310008, [2]浙江省农业科学研究院,杭州310008, [3]河南科技学院,河南新乡453700
  • 相关基金:国家自然科学基金自助项目(31171862); "十二五"农村领域国家科技计划课题(2011BAD01B02-5); 浙江省科技厅公益技术研究农业项目资助(2011C22043)中国计量学院实习生胡子卫、孔繁渊、长江大学实习生杨庭宪以及东北农业大学实习生王晓鹏4名同学参与了多酚氧化酶活性的部分测定工作,谨致谢忱.
中文摘要:

以温室中培养的龙井43茶树2年生扦插苗为实验材料,采用分光光度计法测定了正常茶树叶片中多酚氧化酶(PPO)活性变化的日节律,以及茉莉酸甲酯(MeJA)处理和机械损伤诱导不同叶位叶片PPO活性的时序变化.结果表明:正常茶树叶片中PPO活性高峰出现在4:00;MeJA处理茶树30 h后,其叶片PPO活性与对照间呈现显著差异,于处理后174 h达到最高峰;半定量RT-PCR检测结果发现,MeJA诱导茶树叶片PPO基因表达量最高峰出现在处理后126 h,比生化测定结果提前了48 h.机械损伤可诱导处理叶及其下叶位叶片PPO活性迅速升高,表现出一定系统性;机械损伤叶片与系统诱导叶片间PPO活性随时间变化的趋势基本平行,但机械损伤叶片中PPO活性始终高于系统诱导叶片.本研究证明,MeJA和机械损伤均可诱导茶树叶片中PPO活性的显著增强,进一步证实茉莉酸(JA)信号转导途径在茶树的直接防御反应中具有重要作用.

英文摘要:

Polyphenol oxidase(PPO) plays an important role in defending herbivorous insects.Our objectives were to illuminate the kinetics of polyphenol oxidase activity of healthy plant,induced by(methyl jasmonate) MeJA,mechanical damage and systemic induction by mechanical damage.2-yr-old Longjing tea plants were used.Circadian kinetics of PPO activity was measured by spectrophotometer.RT-PCR was used to confirm the kinetics of polyphenol oxidase gene expression quantity of MeJA treated samples,in order to give proof of the bio-chemistry results.The activity peak occurred at 4 am in intact tea leaves.The activity of PPO was significantly different between MeJA treated leaves and controls at 30 h,mounted the peak at 174 h.Results of RT-PCR showed that gene expression quantity mounted the peak at 126 h,about 48 h ahead of enzymatic activity analysis.Mechanical damage induced PPO activity significantly increase not only in treatment leaf,but also in lower position leaf,which showed systematic reaction.PPO activities were similar in intact leaves in different position.Following mechanical demage,PPO activity increased dramatically and systemically.In systemically wounded leaves,PPO activity changed in parallel to the induction observed in wounded leaves generally,but the increase was consistently slower.Our results showed that elevation of PPO activity could be significantly induced by exogenous application of MeJA and mechanical damage,suggesting that JA signaling pathway may also play an important role in wound-induced plant defense responses in tea plants.

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期刊信息
  • 《西北植物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国教育部
  • 主办单位:西北农林科技大学 陕西省植物学会
  • 主编:赵忠
  • 地址:陕西杨陵邰城路3号西北农林科技大学
  • 邮编:712100
  • 邮箱:xbzwxb@vip.163.com
  • 电话:029-87082936
  • 国际标准刊号:ISSN:1000-4025
  • 国内统一刊号:ISSN:61-1091/Q
  • 邮发代号:52-73
  • 获奖情况:
  • 全国优秀期刊,中国自然科学核心期刊,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:46104