中文摘要：

在单因素试验的基础上,通过正交试验对CgSO1、CgSB2、CgO5和SPOP2这4株土生空团菌[Cenococcumgeophilum Fr.（Cg）]的培养基配方分别进行了优化.结果发现,来源不同的Cg菌株对培养基组分需求存在一定差异,碳氮源、微量元素、无机盐以及维生素都不相同,CgSO1、CgSB2、CgO5对葡萄糖和麦芽汁的混合C源以及牛肉浸膏利用最好,SPOP2对麦芽汁以及酒石酸铵的利用最好,适量的微量元素、无机盐以及维生素B1会促进Cg的生长.经平板培养基优化后的培养基配方可应用到液体培养中,这4株Cg菌经液体培养后菌丝产量分别达到了13.2、12.4、18.9和15.1g/L,均可满足液体发酵的生产要求.

英文摘要：

Based on the results of the single-factor tests, the medium components of four strains of Cg （CgSO1, CgSB2, CgO5 and SPOP2） were optimized by the orthogonal tests. The results showed that the four strains of Cg required different medium components and different carbon sources, nitrogen sources, microelements, mineral salt components and vitamins. The best carbon source and nitrogen source for CgSO1, CgSB2 and CgO5 were glucose ＋ wort and beef extract while SPOP2 were wort and ammonium tartrate. The appropriate amount of microelements, mineral salts and Vitamin B1 enhanced the growth of CgSO1, CgSB2, CgO5 and SPOP2. The results of the optimized medium by plate culture could be used in liquid culture and the biomass of the four strains in liquid culture were 13.2, 12.4, 18.9 and 15.1 g/L, respectively, which all met the requirement of liquid fermentation production. Fig 1, Tab 6, Ref 17