中文摘要：

目的探讨高渗培养基对椎间盘内髓核组织及髓核细胞活力的影响。方法 6-7周龄SD大鼠10只,无菌取每只大鼠胸腰段椎间盘9个,置于高渗（410 mOsm/kg）培养基中整体培养,培养前及培养第7,14,21,28天,利用Mitotracker Green荧光探针、免疫组织化学和生物化学方法检测细胞的活力、椎间盘结构的变化以及髓核组织细胞外基质成分的变化。结果椎间盘组织形态、细胞存活率及髓核细胞外基质成分与新鲜离体椎间盘无统计学差异。髓核细胞存活率荧光强度检测提示培养后第21天与培养前比较荧光强度明显降低（7 782±367 vs 10 435±376,P〈0.05）。髓核组织内蛋白多糖含量（mg/100mg）检测提示培养第21天与培养前相比明显降低（3.72±0.45 vs 6.35±0.76,P〈0.05）。结论在高渗培养基中（410mOsm/kg）,髓核细胞可以良好存活至少14 d,大鼠髓核组织内蛋白多糖可以有效保持14 d。

英文摘要：

Objective To explore the effects of hypertonic medium on cell viability and metabolism of nucleus pulposus in the intervertebral discs. Methods Nine thoracolumbar intervertebral discs were harvested from every 6- 7-week-old SD rat（n = 10）and cultured with hypertonic medium（410 mOsm / kg）. Cell viability,structural integrity and proteoglycan content were assessed using Mitotracker Green fluorescent probe,histochemical and biochemical methods at days 0,7,14,21 and 28 after the culture. Results The morphology of the whole intervertebral discs,cell viability and extracellular matrix component of nucleus pulposus at day 14 showed no statistical changes compared with those in fresh intervertebral discs in vitro. However,cell survival fluorescence intensity decreased significantly at day 21 in comparison of that before culture（7 782 ± 367 vs 10 435 ± 376,P 0. 05）. The proteoglycan content of nucleus pulposus（mg / 100 mg）at day 21 markedly reduced in comparison with that before culture（3. 72 ± 0. 45 vs 6. 35 ± 0. 76,P 0. 05）.Conclusion Nucleus pulposus cells in the rat intervertebral discs cultured with the hypertonic medium（410 mOsm / kg）could survive at least 14 d. Proteoglycan in nucleus pulposus tissue of rats can effectively keep the richness in 14 d.