中文摘要：

目的研究生长分化因子（GDF5）在诱导脂肪干细胞（ADSCs）成软骨分化中的作用。方法大鼠ADSCs在不同诱导条件下经细胞微团培养后,通过检测细胞增殖、糖胺聚糖和蛋白聚糖、软骨细胞特异性基因CollagenⅡ、AggrecanmRNA和蛋白质表达,以及软骨细胞肥大标志基因CollagenⅠ、CollagenⅩ的表达水平,比较不同浓度的GDF5（10ng/ml、100ng/ml和1000ng/ml）与TGFβ1（10ng/ml）对ADSCs成软骨分化的促进作用。结果 GDF5对AD-SCs成软骨分化过程中的细胞增殖具有显著的促进作用,同时还能增加细胞外基质糖胺聚糖和蛋白聚糖的含量,以及上调软骨细胞特异性CollagenⅡ和Aggrecan基因和蛋白表达。在10ng/ml、100ng/ml和1000ng/ml的GDF5剂量组中以100ng/ml为最佳浓度,其效果显著优于10ng/ml的TGFβ1。结论本研究结果表明GDF5能够显著促进脂肪干细胞的成软骨分化,并且以100ng/ml的效果最为明显。

英文摘要：

Objective To study the effects of GDF5 on promoting chondrogenesis of ADSCs.Methods Cell proliferation,glycosaminoglycan and proteoglycan,the mRNA and protein expression of chondrocyte marker genes collagen Ⅱ and aggrecan,and the expression of the marker genes of chondrocyte hypertrophy collagen Ⅰ and collagen Ⅹ were determined in the ADSCs under different induce conditions in micromass culture system.The promoting chondrogenic effects of 10ng/ml,100ng/ml,1000ng/ml GDF5 were compared with those of 10ng/ml TGFβ1.Results GDF5 could promote the proliferation of ADSCs cells in the process of chondrogenesis,increase the contents of extracellular matrix glycosaminoglycan and proteoglycan,and up-regulate the genomic and proteinic of chondrocyte marker genes collagen II.100ng/ml GDF5 was the optimal concentration among 10ng/ml,100ng/ml and 1000ng/ml groups.Conclusion The results of this study demonstrates that GDF5 could promote ADSCs chondrogenesis significantly,and 100ng/ml GDF5 was the optimal concentration.