香蕉的栽培品种绝大多数是三倍体,常规育种难度大,工作周期长,难以采用传统的育种方法进行遗传改良,因此转基因技术成为改良香蕉种质的有效方法。根癌农杆菌介导的香蕉的遗传转化从20世纪90年代起取得的了较大的进步,但仍然存在着很多问题。文章分析了影响农杆菌介导香蕉转化的几个重要环节,并对其研究现状、存在的问题及应用前景作简要概述。
The development of improved Musa by conventional breeding remains to be a difficult endeavor because of the long generation times, various levels of ploidy, sterility of most edible cultivars and limited genetic variability. Therefore, genetic engineering may offer an alternative method for crop enhancement. The transformation system mediated by Agrobacterium tumefaciens has been used successfully to transform banana during the past 10 years. This article reviews recent progresses on transformation of banana mediated by Agrobacterium tumefaciens and summarizes the factors affecting transformation efficiency. Existing problems and its prospects are briefly discussed.