中文摘要：

研制经济方便的新型乙肝植物口服疫苗是全球控制乙型肝炎的现实要求。研制乙肝植物口服疫苗遇到的一个主要的困难是HBsAg基因在受体植物中的表达量低。香蕉是植物口服疫苗理想的受体。因此，以香蕉为受体，进一步提高HBsAg基因表达量的研究非常必要。从pBIL2载体上克隆了香蕉果实特异表达的BanLec启动子．并引入了Hindm、BarnH Ⅰ酶切位点；利用EcoRⅠ和Barn HⅠ双酶切YEPFLAG—HBS获得了HBsAg基因；通过BamHⅠ酶切位点，利用T4DNA连接酶连接了BanLec启动子和HBsAg基因，形成BanLec—HBsAg连接片段：再把该片段通过EcoRⅠ、HindⅢ酶切位点插入pCAMBIA2300植物表达载体，成功构建香蕉果实特异表达启动子驱动下的乙肝表面抗原基因表达载体。

英文摘要：

There is a high demand for development of new convenient edible plant vaccines against hepatitis B. At present, low expression of HBsAg gene in plant is limiting the development of edible plant vaccine against hepatitis B. Banana is regarded as the most ideal plant receptor for edible vaccine.Improving the expression level of HBsAg is essential in banana.In this study, we cloned a banana fruit specific promoter （BanLec）added to Hind Ⅲ and BamH Ⅰ from pBIL2 vector; we digested the YEPFLAG-HBS vector with EcoR Ⅰ and BarnH Ⅰ to gain HBsAg gene; we connected a banana fruit specific promoter （BanLec） to HBsAg gene by BamH Ⅰ to produce a BanLec-HBsAg segment.The segment was inserted into PCAMBIA2300 by EcoR I and HindⅢ. We successfully constructed plant expression vector for HBsAg gene driven by BanLec.