中文摘要：

目的 探讨致死剂量全身照射（TBI）对小鼠T淋巴细胞亚群分布及其细胞因子表达水平的影响.方法 将小鼠随机分为TBI组和未照射对照组.于致死剂量^60Co照射后第7天分离小鼠骨髓、肠系膜淋巴结、脾脏及肝脏的淋巴细胞,用流式细胞术检测细胞免疫表型及细胞内IFN-γ和IL-17表达水平.结果 ①TBI小鼠骨髓、脾、淋巴结及肝脏的淋巴细胞总数低于对照组[（5.34±1.14）×10^5对（3.08±1.13）×10^7、（2.10±0.54）×10^5对（2.71±0.83）×10^7、（5.89±1.07）×10^5对（7.92±1.15）×10^7、（3.45±1.01）×10^5对（7.44±0.79）×10^6,P值均＜0.05];②TBI组小鼠脾、淋巴结及肝组织IFN-γ CD4^＋T细胞百分比高于对照组[（20.77±2.03）％对（3.69±3.13）％、（6.28±0.46）％对（1.11±0.17）％、（27.24± 5.79）％对（9.01±1.24）％,P值均＜0.05];③TBI组小鼠脾、淋巴结及肝组织IFN-γ^＋ CD8^＋T细胞百分比亦高于对照组[（52.40±9.26）％对（43.06±1.04）％、（33.56±5.02）％对（21.83±4.22）％、（44.27±8.97）％对（19.32±3.11）％,P值均＜0.05];④TBI组小鼠脾及肝脏中CD4^＋T及CD8^＋T细胞IL-17A表达水平较对照组降低.结论 TBI可导致小鼠各脏器淋巴细胞数量减少、诱导IFN-γ表达阳性的效应性Th1和Tc1细胞百分比增加.

英文摘要：

Objective To investigate the effects of letherally total body irradiation （TBI） on distribution of T-lymphocyte subtypes and their cytokine expression.Methods The BALB/c mice were divided randomly into 60^Co gamma rays TBI group and control group.Mice were sacrificed 7 days after irradiation.The lymphocytes in spleens,mesenteric lymphonodes,livers and bone marrow were collected and counted.Changes of CD4^＋ T and CD8^＋ T cell subsets as well as the expressions of IFN-γ and 1L-17 were analyzed by flow cytometry.Results ① Compared with control group,the total number of lymphocytes in marrow,spleen,lymph node and liver distinctively decreased in TBI group [（5.34± 1.14）× 10^5 vs （3.08±1.13） × 10^7,（2.10±0.54） × 10^5 vs （2.71±0.83） × 10^7,（5.89±1.07） × 10^5 vs （7.92±1.15） × 10^7 and （3.45±1.01） × 10^5 vs （7.44±0.79） × 10^6,respectively,and the significant differences were observed between two groups in each organ （P〈0.05）].②The level of IFN-γ produced by CD4^＋ T in spleen,lymph node and liver elevated in TBI group compared to control group,which were （20.77±2.03）% vs （3.69±3.13）%,（6.28±0.46）% vs （1.11±0.17）%,（27.24±5.79）% vs （9.01±1.24）% respectively,the differences between two groups in each organ were significant （P〈0.05）.③Percentages of IFN-γ^＋CD8^＋ T in spleen,lymph node and liver in TBI group increased compared to control group [（52.40± 9.26）% vs （43.06± 1.04）%,（33.56 ± 5.02）% vs （21.83 ± 4.22）%,and （44.27 ± 8.97）% vs （19.32 ± 3.11）%,respectively,and the differences between two groups in each tissue were significant （P〈0.05）].④However,IL-17A expressions in CD4＋ T and CD8 ＋ T cells from spleen and liver were lower than those in control group.Conclusion TBI induced the reduction of lymphocytes and the expansion of IFN-γ producing Th 1 and Tc 1 effector cells in mice.