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中文摘要：
通过OTC试验研究了生长60和120 d的转Bt水稻克螟稻及其对照亲本秀水11在2种CO2浓度（375、750μL·L-1）处理下的生理变化。结果表明：叶绿素a、叶绿素b、叶绿素a＋b、可溶性蛋白含量以及谷氨酰胺合成酶活力等均随水稻生长而显著提高,游离氨基酸含量随水稻生长而显著下降,叶绿素a/b值在高CO2浓度下显著增加;CO2浓度升高会引起水稻组织含水量、叶绿素a和c含量显著降低,叶绿素b含量呈先增加后降低的趋势;同时,可溶性蛋白含量显著下降,游离氨基酸含量显著增加,谷氨酰胺合成酶活性初期变化不显著、后期显著降低;转Bt水稻及其对照亲本之间可溶性蛋白含量和谷氨酰胺合成酶活力差异显著,但叶绿素和游离氨基酸含量差异不显著;与亲本相比,不同CO2浓度处理下克螟稻的谷氨酰胺合成酶活力显著降低。
英文摘要：
The physiological responses in transgenic Bt rice expressing Cry1Ab（ KMD） and in the isogenic non-transgenic rice（ XSD） to elevated CO2（ 750 μL·L-1） were studied after planting for 60 and 120 days in open-top chambers（ OTC）. The results showed that rice chlorophyll a,chlorozphyll b,chlorophyll a ＋ b,soluble protein content and glutamine synthetase activity increased from 60 to 120 days,while the content of free amino acids decreased significantly. The value of chlorophyll a / b significantly increased with the elevation of CO2 level. Elevated CO2 significantly reduced the contents of water,soluble protein,chlorophyll a and c in rice foliar tissue,and chlorophyll b content significantly increased at 60 days but significantly decreased at 120 days. The content of soluble protein decreased significantly and that of free amino acids increased significantly. Moreover,the glutamine synthetase activity wasn’t affected by elevated CO2 at 60days,while it was significantly reduced at 120 days. There were significant differences in soluble protein content and glutamine synthetase activity but not in contents of chlorophyll and free amino acids between transgenic Bt rice and non-transgenic rice. Compared to non-transgenic rice,the glutamine synthetase activity of transgenic rice was significantly lower under the ambient or elevated CO2 treatments at 60 and 120 days.
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