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中文摘要：
褐飞虱是水稻的重要害虫，而Wolbachia是其重要的次生共生细菌之一。为了解褐飞虱种群感染Wolbachia的情况，本研究对采自世界褐飞虱主要分布区的22个地理种群感染Wolbachia的情况进行了检测和分析。qPCR结果表明，除杭州2009年种群没有检测出Wolbachia外，其它21个种群中都有不同程度的Wolbachia感染。对Wolbachia的wsp基因序列的比较和进化分析表明，褐飞虱各地理种群Wolbachia都属于B群，并可进一步分为Ori和Con两个亚群。其中18个褐飞虱种群中的Wolbachia属于Ori亚群，广东清远和浙江桐乡褐飞虱种群中的Wolbachia则属于Con亚群，而菲律宾Iloilo褐飞虱种群分别检测到同时有2个亚群Wolbachia感染。本结果可为进一步研究褐飞虱与共生菌的共生关系、褐飞虱致害性变异和迁飞路线分析及利用Wolbachia防治褐飞虱提供依据。
英文摘要：
The brown planthopper （BPH）, Nilaparvata lugens Stal, is one of the most important rice pests, and Wolbachia is a secondary endosymbiont bacteria found in it. To better understand Wolbachia infection in BPH populations around the world, Wolbachia was collected and analyzed from 22 BPH populations in China, India and Southeast Asian Countries. Real-time quantitative PCR results showed that all populations, except that from Hangzhou, were infected with Wolbachia to varying degrees. Sequence alignment and evolutionary analysis of Wolbachia WSPs showed that the Wolbachia from all detected BPH populations belonged to the Wolbachia B group, and could be further divided into two subgroups, Ori and Con. Wolbachia from most of the sampled BPH populations belonged to Ori; only those from Qingyuan and Tongxiang belonged to Con. Interestingly, both Ori and Con were detected in the BPH population from Iloilo, in the Philippines. This work may provide useful data for further research into the symbiosis between BPH and its endosymbionts, BPH virulence change, migration routes of BPH and on the application of Wolbachia to BPH control.
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