中文摘要：

实验被进行决定先进种痘和中国草药的助手(CHA ) 的效果，包含距骨多糖(APS ) 和到兔病毒性出血症(RHD ) 的免疫反应上的人参皂甙(GS ) 在小兔子疫苗。在实验 1，在年龄的 30， 35， 40，或 45 天的每个组的 5 只新西兰兔子分别地与使失去活性的 RHD 疫苗的 2 mL 被注射。抗体乳头 ers 的动态变化被红血球凝聚抑制测试(嗨) 方法。在实验 2，在 35 天年龄的 30 只新西兰兔子随机被分到 5 个处理组，代表有非辅助的 RHD 疫苗的 3 mL 的接种， CHA-RHD 疫苗， CHA -- 哈疫苗(半剂量抗原) ，铝 adjuvant-RHD 疫苗，和 PBS 分别地。外部淋巴细胞增长和浆液抗体乳头 ers 的动态变化被 MTT 方法和 HI 方法测试。结果证明效价母亲嗨，在 35-day-old 兔子的抗体在天是比 3 log2 的保护的水平低的 7 ～ 49 在种痘以后，抗体乳头 ers 比 3 log2 高。CHA 支持了淋巴细胞增长并且提高了浆液抗体效价(P 【 0.05 ) 。从二个实验的这些调查结果建议先进种痘和中国草药的助手显著地对 RHD 提高了免疫反应到疫苗，并且有效地保护了小兔子免于 RHD 挑战。

英文摘要：

Experiments were conducted to determine the effects of advanced vaccination and Chinese herbal adjuvants （CHA）, containing astragalus polysaccharides （APS） and ginsenosides （GS） on the immune response to rabbit hemorrhagic disease （RHD） vaccine in young rabbits. In experiment 1, 5 New Zealand rabbits of each group at 30, 35, 40, or 45 days of age were injected with 2 mL of inactivated RHD vaccine, respectively. The dynamic changes of antibody titers were tested by the hemagglutination inhibition （HI） method. In experiment 2, 30 New Zealand rabbits at 35 days of age were randomly assigned to 5 treatment groups, representing inoculation with 3 mL of non-adjuvant RHD vaccine, CHA-RHD vaccine, CHA-HA vaccine （half dose antigen）, aluminium adjuvant-RHD vaccine, and PBS, respectively. The dynamic changes of peripheral lymphocyte proliferation and serum antibody titers were tested by the MTT method and the HI method. The results showed that the titer of maternal HI antibody in the 35-day-old rabbits was lower than the protective level of 3 log2, while on days 7 to 49 after the vaccination, the antibody titers were higher than 3 log2. The CHA promoted the lymphocyte proliferation and enhanced the serum antibody titer （P〈 0.05）. These findings from the two experiments suggested that advanced vaccination and Chinese herbal adjuvants significantly enhanced the immune response to vaccine against RHD, and effectively protected the young rabbits against RHD challenge.