中文摘要：

采用荧光光谱法研究了β-环糊精（β—CD）、甲基-β-环糊精（M—β-CD）、羟丙基-β-环糊精（HP—β—CD）、磺丁基-β-环糊精（SBEp—CD）对维生素B1的包合作用。在固定维生素B1浓度和改变环糊精及其衍生物浓度的情况下，维生素B1的荧光发射波长的变化以及荧光强度的增强表明了包合物的形成，用荧光双倒数法计算了环糊精及其衍生物与维生素B1的包合常数。实验结果表明：在pH=7．4的体系中，β-环糊精对维生素B1的包合能力最强，且四种环糊精与维生素B1的包合物的包合比均为1：1。

英文摘要：

The inclusion interactions of VB1 with β-cyclodextrin（β-CD）, methyl-β-cyclodextrin（M-β-CD）, hydroxyproyl-β-cyclodextrin （HP-β-CD） and sulfobutyl-β-cyclodextrin （SBE-β-CD） were investigated using steady state fluorescence spectroscopy at 20℃ in pH = 7. 4 medium. Remarkable fluorescence emission enhancement upon addition of VB1 and changes of its emission wavelengths in the presence of CDs suggested the formation of complex. And the formation constants of VB1-CDs were determined by fluorescence spectrometric method, which showed that β-CD had the strongest inclusion capacity with VB1 in pH=7.4 buffer solution. The inclusion ratio for the four CDs with VB1 was all 1=1.