中文摘要：

目的：研究乳腺癌肿瘤相关成纤维细胞（carcinoma-associated fibroblasts,CAFs）对乳腺癌细胞增殖、凋亡、黏附和侵袭能力的影响.方法：首先,从6例乳腺癌病人肿瘤及癌旁正常乳腺组织块分别分离培养成对的CAFs和正常成纤维细胞（normal fibroblasts,NFs）.然后,通过Transwell小室将CAFs或NFs与乳腺癌MDA-MB-231细胞体外共培养,进行增殖（MTT）、侵袭、黏附实验及流式细胞仪细胞凋亡检测.结果：MDA-MB-231与CAFs或NFs共培养后乳腺癌细胞的增殖活性显著增强（P＜0.05）.与NFs相比,这种差异在同CAFs共培养组更为明显（P =0.011）.与CAFs共培养之后,MDA-MB-231的早期凋亡显著减少（P=0.026）;而与NFs共培养之后则无显著差异.CAFs或NFs对MDA-MB-231细胞的细胞周期和晚期凋亡无明显影响.与CAFs或NFs共培养24小时后,MDA-MB-231细胞的侵袭、黏附能力明显增强,黏附数目为（34.7±4.84）个/视野（CAFs）和（20.16±0.09）个/视野（NFs）,（P =0.000）;侵袭数为（89±4.62）个/视野（CAFs）和（81.6±6.08）个/视野（NFs）,（P＜0.05）.结论：CAFs能促进乳腺癌MDA-MB-231细胞的增殖、减少其早期凋亡;同时对乳腺癌细胞的黏附和侵袭能力有促进作用.可是NFs的作用较CAFs为弱.有关CAFs影响乳腺癌肿瘤细胞的生物学特性的机制有待于进一步研究.

英文摘要：

Objectlve：To study the effect of carcinoma -associated fibroblasts （CAFs） on the proliferation, apop- tosis, adhesion and invasiveness ability of MDA - MB - 231 cell. Methods： Fisrstly, culturing paired CAFs and normal fibroblasts（ NFs）isolated respectively from the tumor and adjacent normal breast tissue from 6 patients with breast cancers. Co - culturing breast cancer MDA - MB -231 cells with CAFs and NFs in Transwell chambers. Proliferation （MTT）, invasiveness, adhesion and cell apoptosis were assayed by flow cytometry. Results：The proliferation of MDA -MB-231cells was significantly increased after co- cultured with CAFs or NFs（P 〈 O. 05 ）. The difference was more obvious when co - cultured with CAFs compared with that of NFs（ P = 0. 011 ）. The early apoptosis of MDA - MB - 231 cell decreased after the co - culture with CAFs（P = 0. 026）. There were no significant changes after co - culture with the NFs. There was no difference in the cell cycle and the late apoptosis of MDA- MB-231 cells co - cultured between NFs and CAFs. The adhesion and invasiveness of MDA - MB - 231 cells was increased after co - culture with CAFs or NFs for 24 hours. The adhesion number was（34.7 ±4.84）/field for CAFs and （20.16 ± O. 09）/field, （P =0.000）. The invasive MDA - MB - 231cells number was（89 ±4.62）/field for CAFs and （81.6 ± 6.08 ） / field for NFs, （ P 〈 0.05 ）. Conclusions： CAFs can promote the proliferation and decrease the early apoptosis of MDA -MB -231 cells. CAFs can also increase the adhesive and invasive abilities of MDA -MB -231 cells, being weaken in the NFs group. However, the mechanism about how CAFs affect the biological behavior of MDA - MB - 231 cells needs further study.