中文摘要：

构建一种能够更好地模拟肝细胞生长环境的体外培养平台。利用先进制造方法中的快速成型技术和微压印技术制作生物反应器和三维支架。将三维支架置于生物反应器中,与蠕动泵、氧和器、储液罐构建肝细胞的体外的动态培养系统。研究培养液流速、氧流量与氧含量的关系并进行参数优化,利用细胞支架复合体进行细胞培养。细胞培养结果显示该系统能够维持肝细胞的正常存活和生长。所设计并实现的动态灌注培养系统,通过调整流速和氧流量,可具有良好的氧合效果和较高的效率,能够更好的模拟肝细胞的体内生长环境。

英文摘要：

This project is aimed to build an in vitro equipment simulating the liver cell culture environment.Bio-reactors and three-dimensional scaffolds were built using the rapid prototyping technology and microimprinting technology.The scafflods were put in the bio-reactors connected with pumps,oxgenator,and culture medium reservoir to construct a perfusion cell culture system.The relation of culture medium flow rate,oxygen flux and oxygen content was investigated.Optimized parameters for the culture of hepatocytes were studied as well.HE and SEM results proved that the liver cells could survive and grow well in the system.The developed in vitro dynamic cell culture system has good oxygenation performance and high efficiency.