研究菌株A在Cd^2+存在条件下的生物矿化行为以及所选用菌株修复Cd^2+污染土壤的效果。结果表明:菌株A的脲酶常数Km为1.231mol/L,Cd^2+浓度为0.595mg/L时,菌株生长良好;随着Cd^2+浓度的增加,细菌生长由促进变为抑制。细菌在Cd^2+污染的水溶液和土壤中仍具有很强的酶活性。游离态Cd^2+能够与细菌新陈代谢产物CO3^2-形成CdCO3不溶晶体沉淀。通过盆栽实验,经过菌株A对Cd^2+污染土壤的处理,有效的降低土壤游离态Cd^2+和植物体内Cd^2+含量。在土壤、水资源处理方面,菌株A在生物矿化固结重金属有着广阔的应用前景。
The behavior of biomineralization of bacteria A in the presence of Cd^2+ and the effect of Cd^2+ polluted soil repaired by bacteria A were investigated. The results show that while the bacteria A had a high urease constant Km of 1.231 mol/L and the Cd^2+ concentration was 0.595 mg/L, the bacteria grew well. The growth of bacteria changed from accelerated to restrained with the increase of Cd^2+ concentration. Bacteria A have high urease activity in Cd^2+ polluted solution and soil. Free Cd^2+ ions can react with CO3^2- induced by bacteria urease to form insoluble CdCO3 crystals. By pot experiment, the Cd^2+ content in soil and Cd^2+ concentration in wheat can be efficiently reduced after the polluted soil was recovered by bacteria A culture medium. The extensive prospect of applying bacteria A to soil and water remediation has been shown.