中文摘要：

选用实验室自培育碳酸盐矿化菌，研究了体系、细菌体体系、细菌分泌物液体系对碳酸钙结晶动力学，晶体形貌影响。研究发现细菌液浓度增加，抑制碳酸钙成核动力学“平台区”由0增加到7．8min；细菌体作为异相成核点加速结晶过程；分泌物抑制晶体成核，并随着与Ca^2＋混合时间的不同，“平台期”延长。球形碳酸钙的产生是由细菌分泌物调控；Ca^2＋同有机质表面—COO^-和C—0结合，并且随着相互间作用程度的增加，球状碳酸钙不规整表面逐步转变为光滑表面。本研究对于微生物诱导碳酸钙的工程性应用如混凝土微裂缝修复、古建筑表面防护处理、微纳米碳酸钙颗粒制备等具有一定指导意义。

英文摘要：

The bacteria cultured in our lab were used in this experiment. We studied on the influence of bacteria system, bacterial bodies system and bacterial secretion system on kinetics of formation of CaCO3 crystalt and morphology of CaCO3 crystal. This work yielded that the concentration of bacteria solution increasing, induc- tion time prolonged form 0 to 7.8min correspondingly. Bacteria bodies accelerated the crystallization process. Bacterial secretion restrained CaCO3 nucleation, and its induction time prolonged as different blended time with Ca^2＋. The result indicated that global CaCO3 crystals were induced by bacterial secretion, and there were nucleation sites —COO^- and C—O in organic substances. Irregular surface of global CaCO3 was converted to regular one when the action enhanced between Ca^2＋ and nucleation sites. The results are useful for engineering application, such as repairing micro-crack on concrete surface,protecting old building surface and producing micron and nano calcium carbonate grains.