中文摘要：

目的 探讨双表法对流感病毒感染小鼠肺组织MyD88及NF-κB P65mRNA表达的影响,探讨双表法的作用机制.方法 将120只昆明种小鼠随机分为12组：模型组10只;正常组10只;利巴韦林对照组10只;解表药（银翘散）高、中、低剂量组各10只;固表药（玉屏风散）高、中、低剂量组各10只;双表法（银翘散＋玉屏风散）高、中、低剂量组各10只.动物造模后正常组和模型组均以等体积的生理盐水灌胃,各中药治疗组分别予固表法（玉屏风散提取物）、解表法（银翘散提取物）、双表法（玉屏风散＋银翘散）、利巴韦林灌胃给药.采用RT-PCR法检测MyD88及NF-κB P65mRNA表达情况.结果 正常组和模型组肺组织中均可检测到MyD88mRNA及NF-κB P65mRNA的表达,但模型组明显高于正常组（P＜0.05）.双表法高剂量组MyD88mRNA及NF-κB P65mBNA表达最低,与双表法中低剂量组、解表法各剂量组、固表法各剂量组、利巴韦林组比较均有显著性差异（P均＜0.05）.结论 流感病毒感染小鼠时激活了MyD88/NF-κBP65信号通路,双表法能较好地抑制MyD88mRNA及NF-κB P65mRNA的表达.

英文摘要：

Objective It is to investigate the effects of double relieving superficies syndrome methods on the expression of MyD88 and NF-κB P65mRNA of influenza viral pneumonia in mice and its possible mechanism.Methods 120 Kunming mice were equally divide into 12 groups in random：model group,normal group,ribavirin control group,low,moderate,high dose of Yinqiaosan groups,low,moderate,high dose of Yupingfengsan groups,and low,moderate,high dose of Yinqiaosan ＋ Yupingfengsan groups,10 mice in each group.After animal models infected with influenza virus were established successfully,each treatment group was gastric perfused daily with respective drug.The normal group and the model group were gastric perfused daily with normal saline.Then the mRNA expressions of MyD88 and NF-κB P65 were measured with Real-time PCR.Results The mRNA expressions of MyD88 and NF-κB could be detected in lung tissue in normal and control groups,and the expression was higher in model group than that in the normal group（P ＜ 0.0％）.The expression in high dose of double relieving superficies syndrome methods group were lower than that of the other groups（P ＜ 0.05）.Conclusion Influenza virus infection activate the signaling pathway of MyD88 and NF-κB P65 in the mice,double relieving superficies Syndrome methods can effectively suppress the expression of MyD88 mRNA and NF-κB P65 mRNA.