中文摘要：

虽然是一个累积升值，长链非编码RNA的关键作用（使lincRNAs）在不同的细胞过程中发挥，我们如何在癌症的功能使lincRNAs知识仍然稀疏。在这里，我们提出一个RNA序列的转录谱的肺腺癌和正常同行的综合景观解开使lincRNAs基因调控规律。与相邻的蛋白质编码基因之间的共表达以前的研究结果相一致，使lincRNAs是典型的有限与邻近基因的表达，而在癌组织和正常组织中发现。通过建立一个基于相关基因表达的数学模型，我们区分一个额外的子集称为“使lincRNAs调控使lincRNAs”，代表其在基因调控中的主导作用。监管使lincRNAs数癌显著高于正常组织，且大多正调控蛋白编码基因的反式。利用基因功能验证，确定监管，lincRNA，Gass，影响其预测蛋白质编码的目标。此外，我们发现了数百个差异表达的调控使lincRNAs与一些癌症相关的使lincRNAs夹杂。我们的综合分析显示，提高监管效果，为使lincRNAs调控使lincRNAs在肺腺癌中起关键作用的研究资源。

英文摘要：

Although there is an accumulating appreciation of the key roles that long intergenic non-coding RNAs （lincRNAs） play in diverse cellular processes, our knowledge of how lincRNAs function in cancer remains sparse. Here, we present a comprehensive landscape of RNA-seq transcriptome profiles of lung adenocarcinomas and their paired normal counterparts to unravel gene regulation rules of lincRNAs. Consistent with previous findings of co-expression between neighboring protein-coding genes, lincRNAs were typically co-expressed with their neighboring genes, which was found in both cancerous and normal tissues. By building a mathematical model based on correlated gene expression, we distinguished an additional subset of lincRNAs termed ＂regulatory lincRNAs＂, representing their dominant roles in gene regulation. The number of regulatory lincRNAs was significantly higher in cancerous compared to normal tissues, and most of them positively regulated protein-coding genes in trans. Functional validation, using knockdown, determined that regulatory lincRNA, GASS, affected its predicted protein-coding targets. Moreover, we discovered hundreds of differentially expressed regulatory lincRNAs with inclusion of some cancer-associated lincRNAs. Our integrated analysis reveals enhanced regulatory effects of lincRNAs and provides a resource for the study of regulatory lincRNAs that play critical roles in lung adenocarcinoma.