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中文摘要：
建立了一种基于毛细管的振荡流反转录聚合酶链式反应（RT-PCR）的微流控装置检测烟草花叶病毒（TMV）的新方法。根据TMV移动蛋白基因设计一对PCR引物,对粗提纯TMV颗粒直接进行RT-PCR。用0.025%小牛血清蛋白（BSA）对反应毛细管内壁进行静力学和动力学钝化,提高了PCR效率。在此微流控装置上进行RT-PCR,流速为70μL/min时,检出限为0.01μg cDNA;可以在17min内成功扩增出179bp的目的DNA片段。
英文摘要：
An oscillatory-flow reverse transcription-polymerase chain reaction（RT-PCR） microfluidics based on a capillary was developed for the detection of tobacco mosaic virus（TMV）.A pair of PCR primers was designed according to the TMV movement protein gene.Direct RT-PCR-amplified products,a 179 base pair（bp） DNA fragment,from TMV particles were obtained.0.025% bovine serum albumin was used for the static and dynamic passivation to improve the PCR efficiency.On this device,the detection limit of 0.01 μg cDNA was achieved at the flow rate of 70 μL/min,and the amplification of 179 bp fragments can be successfully performed within 17 min.
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