中文摘要：

目的：探讨虾青素（astaxanthin,AST）对过氧化氢（hydrogenperoxide,H2O2）诱导人视网膜色素上皮细胞（retinal pigmentepithelial cells,RPE）氧化损伤的保护作用。方法：人RPE细胞系传代培养,MTT检测细胞活力,流式细胞仪检测细胞凋亡率,透射电镜观察超微结构变化。结果：MTT结果显示用10-8mol/L和10-4mol/L AST处理后,RPE细胞活性分别提高到53.66%±3.25%和70.43%±2.38%,与氧化损伤组（38.76%±3.74%）比较,差异具有统计学意义（P〈0.05）;流式细胞计数结果显示,预先给予10-8mol/L和10-4mol/L的AST作用后,RPE细胞凋亡率分别下降到30.23%±1.91%和12.58%±2.12%,与氧化损伤组（42.50%±1.94%）比较,差异具有统计学意义（P〈0.05）。电镜观察结果显示,伴随AST作用浓度的增加,细胞形态亦逐渐得到改善。结论：AST可以抑制H2O2诱导的人RPE细胞的凋亡,从而为寻求有效的防治视网膜损伤的药物提供可靠的实验±据。

英文摘要：

AIM ： To investigate the protective effect of astaxanthin（ AST） on human retinal pigment epithelial（ RPE） cells against oxidative damage induced by hydrogen peroxide（ H2O2）.METHODS： Human RPE cells were subcultured,cell activity was detected by MTT, rate of apoptosis was detected by flow cytometry and cell ultrastructure changes were observed under transm ission electron m icroscope.RESULTS： MTT results showed that cell activity elevated to（ 53. 66% ± 3. 25% and 70. 43% ± 2. 38% after10-8m ol / L and 10-4mol / L AST treated. The difference had statistically significant（ P〈0. 05） compared with oxidative injury group（ 38. 76% ± 3. 74%）. Flow cytometry results showed that the apoptosis rate of RPE cells decreased to30. 23% ± 1. 91% and 12. 58% ± 2. 12% in AST pretreated group,the difference was significant（ P〈0. 05） com pared with oxidative injury group（ 42. 50% ± 1. 94%）; Electron m icroscopy show ed that the morphology of cells gradually im proved accompanied with the concentration of AST elevated.CONCLUSION： AST may inhibit hydrogen peroxide-induced apoptosis of RPE cells,it can provide reliable evidence for pursue effective medicine to prevent and treat retina injury.