中文摘要：

目的 探讨17-β雌二醇对H_2O_2诱导氧化损伤的晶状体上皮细胞的保护作用。方法 将体外培养的晶状体上皮细胞分为4组;空白对照组：以正常培养液培养;H_2O_2损伤组：给予培养融合状态达到80%的晶状体上皮细胞每孔加入100μmol·L~（-1）H_2O_2,作用12 h;17-β雌二醇低浓度组：用10μmol·L~（-1）17-β雌二醇孵育细胞24 h后,加入H_2O_2作用12 h;17-β雌二醇高浓度组：用100μmol·L~（-1）17-β雌二醇孵育细胞24 h后,加入H_2O_2作用12 h。通过CCK-8法测定细胞活力,流式细胞仪检测细胞凋亡率,荧光探针标记法测定细胞内活性氧自由基（reactive oxygen species,ROS）,使用721 D分光光度计测定细胞内过氧化氢酶（catalase,CAT）、超氧化物歧化酶（superoxide dismutase,SOD）及谷胱甘肽过氧化物酶（glutathione peroxidase,GSHPx）含量。结果 H_2O_2损伤组晶状体上皮细胞经氧化损伤处理后,细胞存活率明显下降,与空白对照组相比差异有统计学意义（P〈0.05）。与H2O2损伤组（59.34%）相比,17-β雌二醇低浓度组和高浓度组晶状体上皮细胞存活率分别提高到67.44%和78.52%,差异均有统计学意义（均为P〈0.05）。H_2O_2诱导损伤后,H_2O_2损伤组晶状体上皮细胞凋亡率为（41.30±3.21）%,空白对照组为（1.67±0.32）%,两组比较差异有统计学意义（P〈0.01）。17-β雌二醇低浓度组和高浓度组晶状体上皮细胞凋亡率分别为（20.97±1.13）%和（14.27±0.90）%,与H_2O_2损伤组比较差异均有统计学意义（均为P〈0.05）。采用H_2 DCFDA荧光探针检测胞内ROS变化情况,H_2O_2损伤组细胞内ROS表达明显升高,DCF荧光信号明显增强,ROS主峰向基线右侧移位;17-β雌二醇低浓度组和高浓度组荧光信号强度逐渐减弱,ROS主峰向基线左侧移位。H_2O_2损伤组晶状体上皮细胞内CAT、SOD及GSH-Px含量均降低,与空白对照组相比差异均有统计学意义（均为P〈0.05）;17-β雌二醇低?

英文摘要：

Objective To investigate the protective effects of 17β-estradiol on human lens epithelial （ HLE ） cells in oxidative damage induced by H2 02 and its involved mechanisms. Methods HLE cells cultured in vitro were collected and divided into 4 groups;cells in negative control group were cultured with normal medium, cells with 80% fusion in H202 damage group was treated with 100 μmol · L-· H202 for 12 h,cells in 17β-estradiol low dose group were incubated with 10 μmol · L-1 17β-estradiol for 24 h then subjected to 100 μmol · L-1H：O2 for 12 h and cells in 17β-estradiol high dose group were cultured in 100 μmol · L-1 17β-estradiol for 24 h then treated with 100 μmol · L-1 H202 for 12h. Next, cell viability was tested by CCK-8 colorimetric assay, while apoptotic rate was detected by flow cytometry, and intracellular reactive oxygen species （ROS） level was detected by I-I2 DCFDA fluorescence probe labeling method, as well as the contents of catalase （CAT）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were determined by 721 D spectrophotometer. Results When compared with the negative control group, the survival rate of HLE cells in the H2 O2 damage group was significantly decreased, and there was significantly different between both groups （P 〈 0.05 ）. Moreover, the survival rate of the 17 ·-estradiol low dose group （67.44%） and high dose group （78.52%） ,was obviously higher than that of the H202 damage group （59.34%）, and the difference was statistically significant （P 〈 0.05 ）. Af- ter H202-induced injury, there was significant difference in the apoptotic rate of HLE cells between the H202 damage group （41.30 ± 3.21 ）% and the negative control group （1.67 ±0. 32）%. In addition,the apoptotic rates of the 17β-estradiol low dose group and high dose group was （20.97± 1. 13）% and （14.27 ±0.90）% respectively,which was statistically different from the H2 O2 damage group （ all P 〈 0.05 ）. H2 DCFDA fluo- rescent labeling te