中文摘要：

采用微波辅助提取（MAE）与液相色谱-串联质谱（LC-MS/MS）技术,建立了同时测定人体指甲中14种抗精神失常及安眠镇静类物质的分析方法。指甲经冷冻研磨后加入内标溶液,用微波辅助提取。以Allure PFPP苯基柱分离,甲醇（含20 mmol/L乙酸铵）-乙酸铵（20 mmol/L）进行梯度洗脱,采用二级质谱多反应监测模式（MRM）检测14种抗精神失常和安眠镇静类物质及其代谢物。目标物在对应含量范围内均线性良好（r~2≥0.990）;检出限为0.1~50 pg/mg;大多数目标物的定量下限为100 pg/mg;日内精密度和日间精密度均不大于7.8%;准确度在±10.16%范围内。对7例服用部分抗精神失常及安眠镇静类药物的患者指甲进行分析,目标物检出情况与用药情况相符。该方法样品处理简便、高效、快速且分析准确、灵敏度高、选择性好,可用于人体指甲中pg/mg级抗精神失常及安眠镇静类物质的定性定量分析。

英文摘要：

A method based on microwave-assisted extraction combined with liquid chromatography - tandem mass spectrometry （MAE/LC -MS/MS） was established for the simultaneous determination of 14 antipsychotic and sedative substances in human nails. About 20 mg of decontaminated and pulver- ized nail samples was microwave-assistedly extracted for twice in the presence of clozapine-d4 used as the internal standard, and the supernatant was determined by LC - MS/MS using the Allure PFPP column with a mobile phase of methanol（ containing 20 mmol/L ammonium acetate） -20 mmol/L am- monium acetate buffer. Multiple reaction monitoring（MRM） mode was used to analyze 14 antipsy- chotic and sedative substances. Good linearities （ re I〉0. 990） over the corresponding range were ob- tained for all the analytes. The limits of detection （LOD） were in the range of 0. 1 - 50 pg/mg and the limits of quantitation（LOQ） were 100 pg/mg for most of the analytes. The deviations of intra- and inter-RSD were not more than 7.8%. Accuracies were within _＋ 10. 16% . Positive results were ob- tained from 7 authentic nail samples, which were in good accordance with the drug administration of the corresponding antipsychotic and sedative substances. The developed method presents a simpli- fied, efficient and rapid procedure for sample preparation, and shows high sensitivity and selectivity, which is suitable for the simultaneous analysis of 14 antipsychotic and sedative substances at pg/mg levels in nail samples.