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In vitro establishment of a highly effective method of castor bean (Ricinus communis L.) regeneration using shoot explants
  • ISSN号:1009-3575
  • 期刊名称:《内蒙古农业大学学报:自然科学版》
  • 时间:0
  • 分类:S567.190.3[农业科学—中草药栽培;农业科学—作物学] S565.6[农业科学—作物学]
  • 作者机构:[1]School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R. China, [2]Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Tongliao 028000, P.R.China, [3]College of Plant Science, Jilin University, Changchun 130062, P.R.China, [4]College of Agriculture, Inner Mongolia University for Nationalities, Tongliao 028000, P,R. China, [5]School of Life Science, Nanjing Agricultural University, Nanjing 210095, P.R. China, [6]School of Life Science, Shandong University, Jinan 25000, P.R.China
  • 相关基金:supported by the National Natural Science Foundation of China (31260336 and 31460353)
中文摘要:

An efficient plant regeneration protocol was established for castor bean(Ricinus communis L.), in which 0.3 mg L–1 thidiazuron(TDZ) induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54(with a 6-d treatment). Modified 1/2 Murashige and Skoog(MS) basal medium supplemented with 440 mg L–1 Ca2+, 0.2 mg L–1 gibberellic acid and 0.1 mg L–1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Furthermore, 1/2 MS media supplemented with 0.2 mg L–1 1-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.

英文摘要:

An efficient plant regeneration protocol was established for castor bean (Ricinus communis L.), in which 0.3 mg L-1 thidiazuron (TDZ) induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 (with a 6-d treatment). Modified 1/2 Murashige and Skoog (MS) basal medium supplemented with 440 mg L-1 Ca2+, 0.2 mg L-1 gibberellic acid and 0.1 mg L-1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Further- more, 1/2 MS media supplemented with 0.2 mg L-11-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.

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期刊信息
  • 《内蒙古农业大学学报:自然科学版》
  • 北大核心期刊(2011版)
  • 主管单位:内蒙古自治区教育厅
  • 主办单位:内蒙古农业大学
  • 主编:苏德毕力格
  • 地址:呼和浩特市昭乌达路306号
  • 邮编:010018
  • 邮箱:
  • 电话:0471-4304787
  • 国际标准刊号:ISSN:1009-3575
  • 国内统一刊号:ISSN:15-1209/S
  • 邮发代号:16-58
  • 获奖情况:
  • 全国高等学校优秀自然科学学报,教育部优秀科技期刊,内蒙古自治区优秀科技期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国北大核心期刊(2008版),中国北大核心期刊(2011版)
  • 被引量:8610