中文摘要：

为了获得毒蛋白含量低的蓖麻转基因新材料,以毒蛋白A链基因被共抑制的转基因蓖麻植株的T0、T1、T2种子为研究对象,对种子中的毒蛋白含量进行测定。结果表明,采用磷酸盐提取法、BCA试剂盒、UVwin5紫外软件V5.1.0、Band Scan蛋白定量分析软件相结合的方法,测定种子中毒蛋白含量,获得了2个共抑制后稳定遗传的材料,即T2-9-1、T2-15,毒蛋白A链含量均为0,毒蛋白B链含量分别为对照通蓖5号的77.63%,83.38%。这2个材料可以作为低毒蛋白含量的蓖麻材料进行推广。

英文摘要：

The purpose of this experiment was to a chain gene toxic protein was co-suppressed in transgenic castor plants T0,T1,T2 seeds were determination of toxic protein content,obtain a low content of toxic protein ricin new transgenic material to lay the foundation. The results were as follows： we used phosphate extraction method,BCA kit method,UVwin5,ultraviolet V5. 1. 0,Band Scan protein quantitative analysis.,after obtained the two co-suppression stable genetic material,nameed T2-9-1,T2-15,A chain toxin protein content were 0. Contrast Tongliao 5 ricin B chain respectively 77. 63%,83. 38%,both materials could be used as low-toxic protein ricin material to promote.