中文摘要：

通过比较不同保存方法对柽柳（Tamarix chinensis）基因组总DNA提取效果的影响，确定合适的野外采集植物样品的保存方法。采用改良CTAB法提取了室温风干、4℃、-20℃、-80℃和硅胶干燥5种保存方法分别保存24h、1周和1月后的柽柳基因组总DNA，结果表明：4℃保存1月后DNA产量最低，为60．35μg/g，-80℃条件下保存24h的材料中提取的DNA产量最高，为246．92μg／g，保存效果最好。但在野外仪器条件不具备的情况下，柽柳可采用室温风干或硅胶干燥保存的方法，1月内材料中DNA降解程度较轻，可以扩增出所需目的片段。

英文摘要：

Through the comparison of different preservation methods in Tamarix chinensis genome DNA, and determine the appropriate preservation method of wild-collected plant samples. The modified CTAB method was used to extract the genome DNA of Tamarix chinensis at room temperature, 4℃, -20℃, -80℃, and silica drying with each treatment for 24 hours, one week, one month, respectively. The results showed that DNA yield was the lowest （60.35 txg/g） after 1 month preservation under the condition of 4℃, and DNA yield was the highest （246.92 μg/g） after 24 h preservation under the condition of-80℃. However, in the wild, when the instruments were not available, Tamarix chinensis can be preserved at room temperature or silica-gel, DNA degradation of materials was slight within 1 month, which can be used for amplify the desired fragment.