中文摘要：

目的：从人大肠实体瘤不同远近距离部位取癌旁组织进行体外培养，探讨人大肠组织体外培养条件，以及不同条件下，培养不同时间与肠隐窝结构的相关性。方法：分别从大肠癌恶性肿瘤患者距瘤体旁边（约2cm），手术切除的近端最远处（≥10cm），以及两者的中间位取得手术标本，运用重复贴壁法进行贴壁培养。结果：运用倒置显微镜观测，用无血清进行培养的组织块存活成活率为73．3％，含10％的胎牛血清培养液进行培养的组织块存活率81．7％，含20％的胎牛血清培养液进行培养的组织块存活率86．7％。HE染色观察，无血清培养的标本14天内，可看到隐窝完整结构，含10％和20％的胎牛血清培养的标本7天内，可看到隐窝完整结构。结论：人大肠组织块的存活时间与血清浓度成正相关，而隐窝完整结构的保持时间与血清浓度呈负相关。

英文摘要：

Objective From the people＇s congress of solid tumor far different distance bowel cancer organization take part the in vitro culture, Explore bowel tissue culture conditions in vitro National People＇s Congress, as well as the different conditions, the rel- evancy of cultivate a different time and bowel fossae structure. Methods Separately from coloreetal cancer patients with malignant tumor from next to tumors （about 2 cm）, Surgical resection of the proximal the most distant（≥10cm）, And both of middle who a- chieved surgical specimens,Repeat on the method used on the train. Results Using inverted microscope,by serum-free medium, the success rate of 73.3% ,by 10% fetal calf serum, the success rate of 81.7%, by 20% fetal calf serum, the success rate of 86.7%, Using HE Dyeing observations, by serum-free medium the crypts were integrated in 14 days, by 10% or 20% fetal calf serum the crypts were integrated in 7 days, Conclusions culturing human coloreetal carcinoma tissue samples by serum-free medium or 10 % fetal calf serum or 20% fetal calf serum, we find it is positive correlation between time to live and serum. The correlated of time to maintain the integrated crypt structure and serum is negative.