中文摘要：

目的：探讨红景天苷（salidroside，Sal）对帕金森病（Parkinson’Sdisease，PD）小鼠黑质多巴胺转运蛋白（dopaminetransporter，DAT）表达的影响。方法：将神经毒素l-甲基-4苯基-1，2，3，6-四氢吡啶（1-methyl-4-phen-yl-1，2，3，6-tetrahydropyridine，MPTP）注入C57BL／6小鼠腹腔内，制备PD模型，Rotarod实验和游泳实验观察小鼠行为学变化，免疫荧光组织化学法检测黑质DAT阳性神经元数目的变化。结果：行为学实验结果显示，Rotarod实验中模型组小鼠在滚轴上运动的时间明显短于正常组（P〈0．01），给予不同剂量的Sal后，小鼠在滚轴上运动的时间有所延长，并呈剂量依赖性（P〈0．05，P〈0．01）。游泳实验结果与Rotarod结果-致，模型组小鼠游泳时间明显短于正常组（P〈0．01），给予不同剂量Sal后，小鼠游泳时间不同程度增加（P〈0．05，P〈0．01）。免疫荧光结果显示，模型组小鼠黑质中DAT阳性神经元的数目明显少于正常组（P〈0．01），而给予不同剂量Sal干预后，小鼠DAT阳性神经元数目的减少有所恢复（P〈0．05，P〈0．01）。结论：Sal可以改善PD小鼠行为协调能力，提高DAT阳性神经元存活的数目，并呈剂量依赖性，表明Sal对多巴胺（dopamine，DA）能神经元具有-定的保护作用。

英文摘要：

Objective： To investigate the effect of salidroside （Sal） on the expression of dopamine transporter（DAT） in the substanti＇a nigra of Parkinson＇s disease （PD）mice. Methods： The PD model were induced by neurotoxin 1-methyl-4- phenyl-1,2,3,6-tetrahydropyridine（MPTP） injected into the peritoneal cavity of mice. Then, the behavioral changes of mice were recorded by Rotarod test and Swim test. The immunofluorescence histochemistry was used to measure the num- ber change of DAT-positive neurons. Results： Behavioral experiment results showed that the time of mouse moving on the roller in MPTP group in Rotarod test was significantly reduced, compared with the saline control group（ P 〈 O. 01 ）. After the administration of different levels of Sal, the time of the mouse moving on the roller was prolonged, by a manner of dose-dependent （P 〈 0.05, P 〈 0.01 ）. The results of Swim test were consistent with the results of Rotarod test, the time of the mouse swimming in MPTP group was significantly reduced comparing with the saline control group（ P 〈 0.01 ）. And after the different level of Sal was injected, the time of the mouse swimming was prolonged （ P 〈 0.05, P 〈 0.01 ）. The immunofluorescence histochemical results showed that numbers of DAT-positive neurons in the substantia nigra were significantly reduced in MPTP group comparing with the saline control group （P 〈 0.01 ）, but after the different levels of Salwere injected, decreasing of D AT-positive neurons were partiaUy reversed（ P 〈 0.05, P 〈 0.01 ）. Conclusion： Sal could improve the behavioral coordination ability and increase the survival of nigral DAT-positive neurons in PD mice by a manner of dose-dependent, suggesting that Sal might have certain protective effect on dopaminergic neurons.