中文摘要：

目的：研究红景天苷（对-羟基苯乙基-B-葡萄糖，p-hydroxyphenethyl-b-D-glucoside，Salidroside，SAL）对1-甲基-4-苯基吡啶离子（1-methy-4-phenylpyridinium，MPP＋）诱导PCI2细胞凋亡的影响及其可能机制。方法：4-甲基偶氮唑蓝（MTT）检测PCI2细胞活性；Hochest 33258染色，观察细胞凋亡的变化；Western blot检测核因子2相关因子2（nuclearfactor-erythroid 2 p45，related factor2，Nrf2）蛋白的表达情况。结果：MPP＋（500μmol／L）作用于PCI2细胞24h后，与正常组比较，细胞存活率降低（53．3％±3．4％，P〈0．01）；细胞内染色质固缩，呈致密浓染。SAL（10，100μmol／L）预处理24h后，细胞存活率增加（60．8±1．9）％和（87．1土1．8）％（P〈0．01）；细胞核固缩明显减少，且具有剂量依赖性关系。此外，MPP＋可使PCI2细胞中Nrf2蛋白表达减少，而SAL预处理后，PCI2细胞中Nrt2蛋白表达水平明显升高。结论：SAL对MPP＋诱导的PCI2细胞凋亡具有浓度依赖性的抑制作用，其作用机制可能与促进Nrf2蛋白的激活有关。

英文摘要：

Objective： To explore the protective effect of p-hydroxyphenethyl-b- D-glucoside （Salidroside） on 1-methy- 4-phenylpyridinium（ MPP~） induced apoptosis in PC12 cells and its possible mechanism. Methods： The cell viability was measured by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyhetrazolium bromide （MTF）. The morphological change of PC12 cells was observed by Hoechst 33258 staining. The expression level of nuclear factor-erythroid 2 p45, related factor 2 （Nrt2） protein was detected by Western blot. Results： Compared with the control group, the ratio of cell survival de- creased 53.3 ＋ 3.4% （P 〈 0.01 ）; eyto-ehromatin concentration; nucleus showing strong staining after MPP＋ treatment with PC12 cells for 24 h. The cell viability was increased to 60.8 ± 1.9%, 87.1 ± 1.8% （P 〈0.01 ） respectively after exposure with 10,100 μmol/L SAL. Cyto-chromatin concentration reduced and showed the relation between quantity and results. Furthermore, MPP ＋decreased the expression level of Nrf2 protein in the cytoplasm of PC12 cells. But compared with MPP＋ treatment group, the expression level of Nrf2 protein in the cytoplasm of PC12 cells was increased after SAL pretreatment. Conclusion： SAL plays a possible neuroprotective role in MPP ＋ induced apoptosis of PC12 cells by a con-centration-dependent manner, and the mechanism of the effects of SAL may be involved in facilitating Nrf2 activation.