中文摘要：

为了便于检测和纯化转染到非肌肉细胞的生肌因子MyoD，同时为能够与转染的其他生肌因子的表达量进行比较，将MyoD编码区克隆在真核表达载体pcDNA3．1（＋）-HA—His。测序表明克隆的MyoD序列正确，并与标签序列构成一个开放阅读框；Westernblot显示在起始密码子前添加kozak序列GCCGCCACC，可使融合蛋白能够以较高水平表达；生肌转化实验表明，融合蛋白MyoD-HA能够有效激活靶基因的表达。

英文摘要：

In order to conveniently detect and purify the myogenic factor MyoD exoexpressed in the nonmuscle cells, and to compare with the expression level of other myogenic factors cotransfected with MyoD, the coding sequence （CDS） of MyoD was cloned and inserted into pcDNA3. I（＋）HAHis, an eukarytic expression vector. Sequencing result confirms that the in sert is correct and forms the same ORF with the HA and its sequences. Furthermore, Western blot shows that the fusion pro tein MyoDHA expresses with a high level for the addition of kozak sequence GCCGCCACC,and myogenic conversion demon strates the fusion protein MyoDHA can activate the target genes effectively.