中文摘要：

本文介绍了一种简便易行的方法,用以在体外建立近似体内实体瘤的肿瘤模型———肿瘤多细胞球模型（multicellular tumor spheroids,MCTSs）。采用液滴重叠法（liquid overlay method）来构建HeLa肿瘤多细胞球模型,并在光镜下对肿瘤多细胞球的生长状况进行观察描述,再应用场发射扫描电镜和透射电镜观察MCTSs对肿瘤多细胞球的外部和内部超微结构及双光子激光共聚焦荧光显微镜对活细胞球进行激光层切,建MCTSs的三维立体形貌。还利用该模型对于表面不同电荷的量子点进行筛选。结果表明,该模型的建立相对简便易行,并具有明确形态学表征,可广泛应用于对抗肿瘤药物和纳米材料进入肿瘤方式和作用机制等研究。

英文摘要：

We present a flexible and highly reproducible method for using 3 dimensional multicellular tumor spheroids（MCTSs） to imitate solid tumor in vivo.We used liquid overlay method to create the Hela cellular spheroids and this model was perfect in simulating the cellular gradient of proliferative status.The external appearance and internal characters of the 3D cultured cells were observed by scanning electron microscopy and transmission electron microscopy.We obtained the tomography of living Hela cellular spheroids and reconstructed the 3D images by multiphoton microscopy.The penetration behaviors of quantum dots and micelles to Hela spheroid and monolayer cells were investigated by multiphoton microscopy,which resembled the process of imaging materials transferring into solid tumors in vivo.Remarkably,our data indicated the 3D culture system and this 3D imaging method can be a potential ＂filter＂ to screen out nanoparticles with better penetration from their inherent properties.All of these approaches allowed the exploration of nanoparticles and chemotherapeutics delivery to solid tumor in vivo to be easily achieved by applying the MCTSs model in vitro.