中文摘要：

目的：观察针刺对胚胎着床障碍大鼠CD4＋CD25＋Foxp3＋Treg细胞的影响。方法：144只孕鼠随机分为对照组（N）、米非司酮组（M）、米非司酮＋针刺组（A）和米菲司酮＋黄体酮组（w），每组随机再分为6d组、8d组和10d组。其中M组、A组和w组妊娠1d给予米非司酮-麻油溶液造模，N组则给予等量麻油溶液。同时，每天下午A组固定并行针刺三阴交和后三里，N组和M组仅固定，w组肌肉注射黄体酮，直到处死当天结束。统计孕鼠胚胎着床数，用流式细胞术检测外周血CD4＋CD25＋Foxp3＋Treg细胞和子宫内膜的CD4＋Foxp3＋Treg细胞的比例，Western blotting和real·timePCR测定着床点的子宫内膜Foxp3的表达。结果：与N组相比，M组的着床胚胎数、外周血CD4＋CD25＋Foxp3＋Treg细胞和子宫内膜CD4＋Foxp3＋Treg细胞的比例、着床点子宫内膜Foxp3蛋白和mRNA的表达均明显下降（P〈0．05）；与M组相比，A组和w组的着床胚胎数、外周血CD4＋CD25＋Foxp3＋Treg细胞和子宫内膜CD4＋Foxp3＋Treg细胞的比例以及着床点子宫内膜Foxp3蛋白和mRNA的表达均有不同程度地升高。结论：针刺改善胚胎着床障碍大鼠的胚胎着床可能与CD4＋CD25＋Foxp3＋Treg细胞的调节密切相关。

英文摘要：

AIM： To observe the effect of acupuncture CD4＋CD25＋Foxp3＋ （forkhead box ≥3）＋ regulatory T-cells （Treg cells） in rats with embryo implantation failure. METHODS： One hundred and forty-four pregnant rats were randomly divided into control group （ N ）, mifepristone treatment group （ M ）, mifepristone ＋ acupuncture treatment group （A） and mifepristone ＋ progestin treatment group （W）. The rats in groups M, A and W were treated with mifepristone- sesame oil solution on day 1, while the rats in group N were injected with the same amount of sesame oil. The Housanli （ST36） and Sanyinjiao（SP6） points were selected for acupuncture. From day 1 to the time of death, the rats in group A were fasten up and then the acupuncture was performed. Accordingly, the rats in group N and group M were only fixed, and the rats in group W were given progestin. Implanted embryos in each group were counted. The proportions of CD4 ＋ CD25＋ Foxp3 ＋ Treg cells in peripheral blood and CD4＋ Foxp3 ＋ Treg cells in the endometrium were detected by flow cytometry. The mRNA and protein levels of Foxp3 were determined by real-time PCR and Western blotting, respectively. RESULTS ： Compared with group N, the number of implanted embryos, the percentages of CD4 ＋ CD25＋ Foxp3 ＋Treg cells in peripheral blood and CD4＋ Foxp3 ＋ Treg cells in the endometrium, and the expression of Foxp3 protein and mRNA in the endometrium were significantly decreased in group M （P 〈 0. 05 ）. Compared with group M, the above indexes in group A and group W were significantly increased. CONCLUSION： The effect of acupuncture in rats with embryo implantation failure may be closely correlated with the modulation of CD4 ＋ CD25＋ Foxp3 ＋ Treg cells. [