中文摘要：

目的观察雌二醇、睾酮和孕酮对3T3-L1脂肪细胞VisfatinmRNA和蛋白表达的影响。方法10娟m01／L～10^-6mol／L雌二醇、睾酮或孕酮作用于3T3-L1成熟脂肪细胞和前脂肪细胞，孵育过夜后收集细胞，分别采用RT-PCR和Westernblot检测VisfatinmRNA和蛋白的表达情况。结果在3T3-L1成熟脂肪细胞．与对照组相比，雌二醇和睾酮分别使VisfatinmRNA表达增加24％（1．74±0．31比1．40±0．18，P〈0．05）和28％（1．65±0．90比1．29±0．69，P〈0．05）；而孕酮不影响成熟脂肪细胞VisfatinmRNA表达。雌二醇轻度增加成熟脂肪细胞Visfa—tin蛋白表达，但无统计学差异；10^-6mol／L睾酮使成熟脂肪细胞Visfatin蛋白表达增加134％（0．61±0．40比0．26±0．05，P〈0．05）。与雌二醇和睾酮不同，孕酮使成熟脂肪细胞Visfatin蛋白表达下调32％（0．19±0．02比0．28±0．02，P〈0．05）。在前脂肪细胞，与对照组相比，10^-7mol／L和10^-6mol／L雌二醇使VisfatinmRNA表达增加70％（1．04±0．38比O．61±0．16，P〈0．01）和123％（1．36±0．41比0．61±0．16，P〈0．01）；睾酮使VisfatinmRNA表达增加76％（1．02±0．24比0．58±0．36，P〈0．05）；孕酮使前脂肪细胞VisfatinmRNA表达增加2．6倍（1．53±1．01比0．42±0．14，P〈0．05）。结论性激素通过促进或抑制脂肪细胞Visfatin基因或蛋白的表达，参与调节上述激素引起的脂肪细胞胰岛素抵抗的病理生理过程。

英文摘要：

Aim To examine the effect of estradiol, testosterone and progesterone on Visfatin gene and protein expression in cultured 3T3-L1 adipocytes and preadipocytes. Methods 0 mmol/L （sex hormones-free DMEM/F12） , 10-8 moL/L, 10-7 mol/L and 10-6 mol/L estradiol or testosterone or progesterone were added to cultured 3T3-L1 adipo- cytes or preadipocytes overnight. Total RNA and proteins were extracted. Then the expression of Visfatin mRNA and protein was measured by RT-PCR and Western blot, respectively. Results Overnight incubation with estradiol in- creased Visfatin mRNA expression in both adipocytes （24% to 21% , P 〈 0. 05 ） at the concentration of 10-s mol/L to 10-7 mol/L and preadipocytes （70% to 123% , P 〈 0.01 ） at 10-7 mol/L to 10 -6 mol/L, respectively. Visfatin mRNA expression increased by 28% （ P 〈 0. 05 ） at 10 -s mol/L testosterone in adipocytes, 76% （P 〈 0. 05） at 10 -7 mol/L testos- terone in preadipocytes. Progesterone did not affect Visfatin mRNA expression on adipoeytes, but had a marked increase in Visfatin expression in preadipocytes by 2. 6-fold （P 〈 0. 05 ）. In 3T3-L1 adipocytes, estradiol had small but insignifi- cant effects on Visfatin protein expression, whereas testosterone had a much greater effect, increasing Visfatin protein by 134% at 10-6 mol/L （P 〈0. 05）. However, progesterone significantly decreased Visfatin protein by 32% at 10-6 mol/L （P 〈0. 05 ）. Conclusions These data suggest that Visfatin may play a physiological role in the insulin resistance caused by estradiol, testosterone or progesterone in 3T3-L1 adipocytes and preadipocytes.