中文摘要：

以十六烷基三甲基溴化铵（CTAB）为模板,正硅酸四乙酯（TEOS）为硅源,合成出纳米介孔二氧化硅（Me-so-SiO2）,并制成修饰碳糊电极.用K3[Fe（CN）6]探针研究了修饰电极的传感特性,介孔二氧化硅不仅显著增加了碳糊电极的表面积,而且明显提高了其异相电子交换速率常数.研究了雌二醇的电化学行为,结果表明介孔二氧化硅对雌二醇的氧化有显著的增敏效应,明显地提高其氧化峰电流.考察了pH值、介孔二氧化硅用量、富集时间对雌二醇氧化信号的影响规律,建立了一种测定雌二醇的新电分析方法,其线性范围为1.0×10^-7－7.0×10^-6mol/L,检测限为6.0×10^-8mol/L.将此方法用于注射液中雌二醇含量的测定,结果与标示量很好地吻合.

英文摘要：

A mesoporous silica（Meso-SiO2） was synthesized using cetyltrimethylammonium bromide（CTAB） as the template,and tetraethyl orthosicate（TEOS） as the silicon source.The obtained Meso-SiO2 was used to modify the carbon paste electrode（CPE）,which characterized using the electrochemical probe of K3[Fe（CN）6].Meso-SiO2 not only remarkably increases the surface area of CPE,but also improves the standard heterogeneous rate constant.The electrochemical behavior of estrogen was investigated,showing that Meso-SiO2 exhibits notable enhancing effect to its oxidation and greatly increases its oxidation peak current.The effects of pH value,amount of Meso-SiO2 and accumulation time were examined,and a novel electrochemical method was developed for the determination of estrogen.The linear range is from 1.0×10^-7-7.0×10^-6 mol/L,and the limit of detection is 6.0×10^-8mol/L.The method was used to detect the content of estrogen in the injection samples,and the results are in good agreement with the labeled content.