中文摘要：

旨在研究β-catenin在不同毛色羊驼皮肤中的表达和定位，探索其与毛色的关系。以成年白色和棕色羊驼为研究对象，采用实时荧光定量PCR技术、Westernblot和免疫组织化学技术，对β-catenin在白色和棕色羊驼皮肤中mRNA、蛋白表达水平和定位进行研究。实时荧光定量PCR结果显示，β-catenin在棕色羊驼皮肤组织中的相对基因表达量是白色羊驼皮肤组织的l_662倍；Westernblot结果显示，羊驼皮肤组织粗蛋白提取物中存在分子量约85ku与兔抗β-catenin多克隆抗体发生免疫阳性反应的蛋白条带，棕色羊驼平均蛋白表达量显著高于白色羊驼；免疫组织化学结果显示，β-catenin在羊驼皮肤的表皮、毛乳头、毛根鞘和皮脂腺中表达，根据光密度值得出，除皮脂腺之外，在表皮、毛乳头和毛根鞘的表达差异极显著（P〈0．01）。结果提示β-catenin在白色和棕色羊驼皮肤组织中定位以及表达的差异，表明β-catenin参与毛色形成。

英文摘要：

The objective of this study was to study the expression and localization of β-catenin in alpaca skin of different colors, and to explore the function of β-catenin in the process of coat color formation. The mRNA and protein expression level of β-catenin in alpaca skin of different colors were examined by Real-time quantitative PCR, Western blot and immunohistochemistry. The gene expressive quantity of β-catenin in the brown alpaca＇s skin tissue was 1. 662 times than ones of white alpaca. The distribution of β-catenin in alpaca skin were demonstrated, the expression was notable difference between the white and brown alpaca＇s based on the average optical density. Our findings showed that that β-catenin was involved in the process of coat color formation.