中文摘要：

旨在研究内皮素-1（Endothelin-1,ET-1）对羊驼皮肤黑素细胞（Melanocyte,MC）增殖和黑素合成的影响。本研究中,体外培养正常羊驼皮肤黑素细胞,观察不同浓度ET-1（0、0.1、1、10、100nmol.L-1）对羊驼皮肤黑素细胞增殖、黑素含量、内皮素受体B（Endothelin recepter B,EDNRB）基因、酪氨酸酶（Tyrosinase,TYR）基因、酪氨酸相关蛋白-1（Tyrosinase related protein 1,TRP-1）基因和表皮黑皮素-1受体（Melanocortin 1receptor,MC1R）基因表达量的影响。结果表明,ET-1处理羊驼皮肤黑素细胞3d后,羊驼皮肤黑素细胞增多,黑素含量、EDNRB、TRP-1和TYR基因表达量都明显增加（P〈0.05）,以10nmol.L-1组最为显著。ET-1能诱导羊驼皮肤黑素细胞增殖、树突增长,诱导EDNRB、TYR和TRP-1基因表达量增高,使黑素合成增加;同时诱导MC1R基因表达量增高,从而通过α-MSH信号通路对羊驼黑色素的生成产生影响。

英文摘要：

To study the effects of Endothelin-1（ET-1） on the proliferation and melanin synthesis of cultured alpaca skin melanocytes,the cultured alpaca skin melanocytes were treated with various concentrations of ET-1（0,0.1,1,10,100 nmol·L-1） in vitro.The effects of ET-1 on melanocyte proliferation,melanin contents,EDNRB,TYR,TRP-1 and MC1R gene expeession in cultured melanocytes were observed.Three days after treatment with ET-1,the number of melanocytes increased and melanin contents,EDNRB,TYR and TRP-1 gene expression levels also remarkably increased in treated cells compared to those in untreated cells（P〈0.05）,especially when the concentration was 10 nmol·L-1.The result indicate that ET-1 can induce the dendrites elongated,proliferation and the expression of EDNRB,TYR and TRP-1,and induce the melanin synthesis;Also,ET-1 induce the expression of MC1R gene,and affect the melanin synthesis of alpaca by the pathway of α-MSH.