For researching the residual stress distribution characteristics of the different large thickness titanium alloy joints by electron beam welding,under heat treatment or non-heat treatment conditions,the changes in residual stress distribution characteristics of electron beam welding joints,which thickness is 50 mm,were measured using blind-hole method with local layer-by-layer removal.Effect of post-welding heat treatment on the residual stress distribution also studied.The results show that blind-hole method with local layer-by-layer removal is suitable to measure residual stresses in thick plate,and good to reflect the welded residual stress distribution of large gradient.Residual stress of the test samples in the weld and heat affected zone has high stress level,and internal stress dramatic changes with thickness in this areas.After heat treatment,the samples’ stress distributions were significantly reduced.The samples’ transverse and longitudinal residual stress are basically the same.Homogenization is obvious.