中文摘要：

【目的】类酵母菌（Yeast-Like-Symbiots,YLS）是存在于褐飞虱（Nilaparvata lugensStl）腹部的一种共生菌,分离获得该共生菌的离体菌株,并鉴定其种属,对于研究褐飞虱致害性变异具有重要意义。【方法】首次运用卵块离体培养的方法、采用改进后的培养基配方,从褐飞虱体内分离到两株共生菌。并运用分子生物学手段,测定离体菌株26S rDNAD1/D2区域序列,分析鉴定菌株种属。【结果】所得菌株与解脂假丝酵母（Yarrowia lipolytica）、嗜盐梗孢酵母（Sterigmatomyces halophilus）的序列相似性分别达到100%和99.8%。【结论】证明褐飞虱体内类酵母共生菌可进行离体培养,并可在人工培养基上存活。同时也验证了褐飞虱菌胞中存在不同种类的类酵母菌,类酵母共生菌为混合物的推测。

英文摘要：

[ Objective ]Yeast-like-symbiots （YLS） is the kind of symbionts exiting in the abdomen of brown planthopper （BPH）, isolated and identified the yeast-like-symbiots of the brown planthopper is very important for studying the virulence variation of the BPH. [Method] Two strains of YLS were isolationed from eggs of BPH （ Nilaparvata lugens Stal）, and which were identified to species level with the 26S rDNA sequences. [ Result] The results showed the cultured strains belongs to the Yarrowia lipolytica and Sterigmatomyces halophilus. [Conclusion] The symbiotes of BPH can be cultured in vitro and survived in artificial culture medium, and the YLS are the complex and which are composed with several different yeasts.