中文摘要：

目的：研究山茱萸活性成分5-羟甲基糠醛对H2O2诱导损伤的大鼠海马神经元凋亡及BCL-2、NF-κB蛋白表达的影响。方法：常规方法进行乳大鼠海马神经元的原代培养,复制体外H2O2氧化损伤模型,采用MTT法分析不同浓度5-羟甲基糠醛对大鼠海马神经元增殖的影响,流式细胞仪检测不同浓度5-羟甲基糠醛对大鼠海马神经元凋亡的影响,Western blotting检测BCL-2、NF-κB蛋白的表达。结果：MTT结果分析表明,模型组与对照组比较,差异显著（P〈0.05）,5-羟甲基糠醛高、中剂量组与模型组相比也有显著性差异（P〈0.05）;流式细胞仪检测结果显示,5-羟甲基糠醛高、中剂量组较模型组细胞凋亡率显著降低;Western blotting检测结果显示,5-羟甲基糠醛高、中、低剂量能提高BCL-2蛋白的表达,并对NF-κB的表达有抑制作用,并呈剂量依赖关系。结论：山茱萸活性成分5-羟甲基糠醛能抑制H2O2诱导的大鼠海马神经元的凋亡,其机制可能与其增加BCL-2蛋白表达,降低NF-κB蛋白表达有关。

英文摘要：

Objective：To investigate the effect of 5-hydroxymethyl furfural（5-HMF） on apoptosis and BCL-2,NF-κB gene expression of rat hippocampal neurons injured by hydroperoxide（H2O2）.Methods： Hippocampal neurons of newly born rat were cultured in vivo and injured by H2O2.Effect of different concentration of 5-HMF on cell viability was measured by MTT.Flow cytometer（FCM） was used to measure the apoptosis of rat hippocampal neurons pre-cultured with different concentration of 5-HMF,Western blotting was used to measure the expression of BCL-2 and NF-κB gene.Results：It revealed that the high and medium dosage of 5-HMF could increase the activity of rat hippocampal.The high,medium and low dosage of 5-HMF also increased the expression of BCL-2 gene and decreased the expression of NF-κB gene.Conclusion：5-HMF could restrain the apoptosis of cultured hippocampal neurons injured by H2O2.The mechanism may be related to increasing in BCL-2 level and decreasing in NF-κB level.