中文摘要：

目的：研究山茱萸不同剂量灌胃家兔后,家兔脑脊液中5-羟甲基糠醛的含量。方法：山茱萸高、中、低剂量连续灌胃家兔3 d后,按常规方法取家兔脑脊液,分别运用HPLC和UPLC-MS法对家兔脑脊液中5-羟甲基糠醛的含量进行检测。结果：采用HPLC法只能在山茱萸水煎液高剂量组家兔的脑脊液中检测出5-羟甲基糠醛,而采用UPLC-MS法,能在山茱萸水煎液各剂量组家兔的脑脊液中检测出5-羟甲基糠醛,并呈一定剂量相关性。结论：5-羟甲基糠醛能透过家兔的血脑屏障,进入到脑脊液中。

英文摘要：

Objective：To detect 5-HMF from rabbit ncurolymph after given different dosage of Cornus officinalis via intragastric administration by HPLC and UPLC-MS.Methods： Rabbit ncurolymph was cramped out three days after given low,medium,and high dosage of Cornus officinalis.5-HMF from rabbit ncurolymph was detected with HPLC and UPLC-MS,respectively.Results：5-HMF from rabbit ncurolymph was detected with HPLC method only in rabbits given high dose group.Meanwhile,5-HMF could be detected with UPLC-MS method in rabbits given medium as well as low dose group.Conclusion：These results suggest that 5-HMF can cross the blood brain barrier（BBB） of rabbits and enter the rabbit ncurolymph.