中文摘要：

目的研究大鼠嘌呤霉素肾病模型中α-parvin的表达变化，探讨其在蛋白尿发生中的作用。方法通过建立嘌呤霉素肾病模型，应用光镜、电镜观察肾脏病理改变，免疫组织化学法检测α-parvin在肾脏的表达与分布。结果（1）嘌呤霉素肾病4d组大鼠24h尿蛋白含量较对照组即已升高（P〈0．01），嘌呤霉素肾病7d组达高峰（P〈0．001）。（2）透射电镜显示嘌呤霉素肾病4d组大鼠足突部分融合，嘌呤霉素肾病7d组大鼠足突广泛融合，嘌呤霉素肾病28d组大鼠足突形态有所恢复。（3）免疫组织化学显示随着α-parvin于肾小球的表达，嘌呤霉素肾病4d组α-parvin的表达较对照组即已升高（P〈0．05）。嘌呤霉素肾病7d组表达明显高于对照组（P〈0．01）。嘌呤霉素肾病28d组α-parvin的表达明显低于嘌呤霉素肾病7d组（P〈0．01）。结论大鼠嘌呤霉素肾病模型中，盯parvin的表达增加可引起足突融合，并与蛋白尿产生密切相关。

英文摘要：

Objective To investigate the possible role of α-parvin in the development of proteinuria in rots with puromyein aminonucleoside nephropathy （PAN）. Methods The PAN model was established by a single injection of puromycin aminonucleoside. The glomerular morphology and podocyte ultrastructure were observed under the light and transmission election mieroscopies respectively. The expression of α-parvin was detected by using immunohistochemistry. Results （1）Pmteinuria was increased 4 days after puromycin aminonucleoside injection and reached a peak on the 7th day（P〈0. 01） ; （2） In the process of the development of PAN, the foot process fusion was observed on the day 4 following puromycin aminonucleoside injection and wild process fusion on the day 7; （3） The expression of α-parvin was significantly up-regulated with the progression of proteinuria, and recovered with the normalization of the urine protein excretion. Conclusions A dramatic alteration of α-parvin protein expression in PAN suggests its potential relationship with the foot process fusion and the development of proteinuria.