中文摘要：

目的探讨血管紧张素Ⅱ（AngⅡ）诱导下大鼠肾脏及条件永生性小鼠足细胞c-Abl的表达变化。方法采用AngⅡ泵（400ng·kg^-1·min^-1）植入sD大鼠皮下的方法建立AngⅡ输注模型，24只大鼠成模后被随机分为AngⅡ输注2周组、AngⅡ输注4周组、AngⅡ输注＋替米沙坦（ARB，3mg·kg^-1·min^-1）干预2周组及4周组，同时设生理盐水输注组和健康对照组，每组6只。分别于成模后2周末、4周末处死大鼠取肾。电镜下观察。肾脏足细胞超微结构的改变；免疫荧光法检测肾脏c-Abl表达；实时PCR及Western印迹检测c-AblmRNA及蛋白水平的改变。对于体外培养条件永生性小鼠足细胞，免疫荧光法检测足细胞肾脏c．Abl的表达，实时PCR及Western印迹法检测足细胞在AngⅡ不同作用浓度（10^-9mol／L-10^-6mol／L）及不同作用时间点（0h、3h、6h、12h、24h）c-AblmRNA和蛋白水平的变化。结果（1）免疫荧光检测结果显示肾脏足细胞有c．Abl表达；实时PCR及Western印迹结果显示AngⅡ输注2周组和4周组大鼠肾脏c-Abl表达增加（P〈0．05），ARB干预组大鼠肾脏c-Abl表达较同期AngⅡ输注组均有降低（P〈0．05）。（2）体外培养的条件永生性小鼠足细胞胞质及胞核有c-Abl表达。AngⅡ可诱导培养的小鼠足细胞c-AblmRNA及蛋白表达增加（P〈0．05），且呈时间和剂量依赖性。结论c-Abl在肾足细胞及体外培养足细胞均有表达，AngⅡ可诱导c-Abl表达上调。c-Abl可能参与了AngⅡ诱导的足细胞损伤。

英文摘要：

Objective To evaluate the effects of angiotensin Ⅱ （Ang Ⅱ）infusion on renal c-Abl expression in vivo, and on podocyte c-Abl expression change in cultured mouse podocytes. Methods Twenty four male Sprague-Dawley rats （Group C, D, E and F） were assigned to receive Ang Ⅱ （400 ng·kg^-1·min^-1） by osmotic minipump and of which 12 rats （Group D and F） were assigned to receive telmisartan （3 mg·kg^-1·min^-1）, six rats received normal saline（Group B）, and six rats were used as normal control （Group A）. Animals were sacrificed at day 14 （Group C and D）, day 28 （Group E and F） respectively. Conditionally immortalized mouse podocytes were used in vitro. Podocytes were studied 2 weeks after thermoswitching from 33℃ to 37℃. Cells were fetal bovine serum （FBS） starved for at least 12 hours prior to stimulation. The cultured podocytes were treated with Ang Ⅱ doses ranging from 10^-9mol/L-10^-6mol/L and for different hours. Expression of renal and podocytes c-Abl was examined by immunofluorescence staining, real-time PCR and Western blotting. Results （1） Distribution of c-Abl expression was mainly in thecytoplasm and nuclear of the podocytes in vivo and in vitro. （2） Expressions of c-Abl mRNA and protein were increased in Ang Ⅱ-infused rat podocytes and Ang Ⅱ-induced cultured mouse podocytes （P〈0.05）, and the effects of Ang Ⅱ were dose-dependent and time-dependent in vitro. Conclusion There are c-Abl mRNA and protein expression in podocytes, and c-Abl may play a critical role in the pathogenesis of Ang Ⅱ-induced podocyte injury.