中文摘要：

背景与目的：观察香加皮杠柳苷（periplocin extracted from cortex periplocae，CPP）对人结肠癌细胞SW480体内外生长的抑制作用。材料与方法：应用MTT法检测CPP不同浓度（0．125、0．25、0．5、1．0、2．0μg／ml）处理SW480细胞后，对细胞增殖的影响；并于CPP（0．5μg／ml）处理SW480细胞24h后用光学显微镜及透射电镜观察细胞凋亡形态学变化；免疫细胞化学检测细胞Survivin蛋白表达变化。采用裸鼠移植瘤模型进行体内移植瘤抑制实验。以上各实验均设立对照组。结果：与对照组比较，CPP对SW480细胞增殖具有明显的抑制作用（P〈0．01），并呈剂量和时间依赖效应；CPP处理SW480细胞后，细胞出现典型的细胞凋亡形态学改变；CPP可明显下调SW480细胞survivin蛋白表达阳性率和表达水平（P〈0．01）；以30mg／kgCPP对SW480细胞裸鼠移植瘤进行治疗后，荷瘤小鼠的肿瘤重量和体积均被明显抑制，抑瘤率为61．41％（P〈0．01）。CPP治疗组小鼠的移植瘤组织出现明显炎性细胞浸润和坏死性变化。结论：CPP对人结肠癌细胞SW480的体内外生长均具有明显抑制作用，其作用机制可能与抑制细胞survivin蛋白表达、诱导细胞凋亡有关。

英文摘要：

BACKGROUND AND AIM： The inhibition effect of periplocin extracted from cortex periplocae （CPP） on proliferation of colorectal cancer cell line SW480 was studied in vitro and in vivo. MATERIALS AND METHODS： The inhibitiory effects of different concentrations of CPP （0. 125,0.25,0.5,1.0, 2.0 μg/ml） on the growth of SW480 cells in vitro were measured by MTT assay. The cell morphology and microstructural changes of apoptosis were examined under light microscope and electron microscope after SW480 cells were treated with CPP（0.5 μg/ml） for 24 h. Survivin expression in SW480 cells was analyzed by immunohistochemistry after cells were treated with CPP （0.5 μg/ml） for 24 h. Model of transplanted tumor on nude mouse were used to study the anticancer effect in vivo. Control group was included in all experiments above. RESULTS： CPP significantly inhibited the proliferation of SW480 cells in a dose- and time-dependent manner in vitro （P 〈 0.01） . After treatment with CPP, SW480 cells showed some typical morphologic features and microstructural changes of apoptosis. And the expression of Survivin in SW480 cells was suppressed. After treatment with CPP 30 mg/kg for SW480 cell tumor-bearing mice, the inhibition rate of transplanted tumor was significantly increased at 61.41%. Inflammatory cells and noticeable necrosis were found in transplanted tumor tissue of CPP treated group. CONCLUSION： CPP had significant inhibitory effect on colorectal cancer cell line SW480. The mechanism maybe associated with CPP inhibiting the expression of Survivin in SW480 cells and inducing cell apoptosis.