中文摘要：

目的:研究腺相关病毒载体(AAV)感染各类正常细胞和肿瘤细胞的效率,为相关研究提供参考。方法:利用脂质体将重组腺相关病毒的包装质粒AAV-DJ、Helper以及表达绿色荧光蛋白(GFP)的穿梭质粒AAV-GFPP转染293FT细胞进行病毒包装,收集病毒液后感染2种正常细胞和14种肿瘤细胞,24 h后倒置荧光显微镜下观察GFP的表达,并随机选取3个视野统计感染GFP的细胞数目。结果:GFP重组腺相关病毒对各种细胞的感染效率存在差别,感染效率最高的为293FT细胞(占96.2%)和Hela细胞(占81.86%),对SCG7901、SEM-M和SP20细胞的感染效率近乎为零。结论:GFP重组腺相关病毒对正常细胞293FT感染效率最高,对肿瘤细胞Hela、SMC7721、BGC823、PANC-1、A549、A875、C6和MCF-7的感染效率较高,这类细胞适合使用AAV作为载体进行外源基因的导入及功能研究。

英文摘要：

Objective:To Study on transfection effection and expression of recombinant adeno-associated virus in kinds of cell lines.Methods:Packaging 293FT cells with the packaging plasmids AAV-DJ, Helper, and shuttle plasmids, AAV-GFP of recombined adeno-associated virus by liposome. Collect the solution of virus and infect 2 normal cells and 15 kinds of cancer cells. 24hours after infection, the efficacy of adenoviral transfered into kinds of cells was assessed by quantification of GFP-positive cells under an inverted fluorescence microscope. Count cells in 3 random sights under microscope.Results:The efficiency of GFP recombinant adeno-associated virus is various in these kinds of cells. The highest efficiency shows in 293FT cells and Hela cells, 96.2% and 81.86%. Conversely the efficiency is approximately 0 in SCG, SEM-M, SP20 cells.Conclusion: Due to the high and relatively high transfection efficiency of the AAV-GFP in normal cells, 293FT, and cancer cells, Hela, SMC7721, BGC823, Panc-1, A549, A875, C6, MCF-7, it is available to do some genetic therapy researh using AAV as a vector.